anti-NS5B (HCV), mAb (5B-3B1)

CHF 400.00
In stock
AG-20B-0002-C100100 µgCHF 400.00
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Product Details
Synonyms Nonstructural Protein 5B
Product Type Monoclonal Antibody
Clone 5B-3B1
Isotype Mouse IgG2b
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a).

Western Blot: (1:1'000)

Crossreactivity HCV

Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity). It does not recognize genotype 2a.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG2b Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.

Product References
  1. Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002)
  2. Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells: R.W. Hardy, et al.; J. Virol. 77, 2029 (2003)
  3. Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs: G. Randall, et al.; PNAS 100, 235 (2003)
  4. Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005)
  5. Quantitative analysis of the hepatitis C virus replication complex: D. Quinkert, et al.; J. Virol. 79, 13594 (2005)
  6. Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3: Y.V. Svitkin, et al.; J. Virol. 79, 6868 (2005)
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