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AdipoGen Life Sciences
anti-NS5B (HCV), mAb (blocking) (5B-12B7)
400
CHF
CHF 400.00
In stock
AG-20B-0003-C100100 µgCHF 400.00
| Product Details | |
|---|---|
| Synonyms | Nonstructural Protein 5B |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 5B-12B7 |
| Isotype | Mouse IgG2a |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase. |
| Application |
Immunocytochemistry: (1:100) |
| Crossreactivity | HCV |
| Specificity |
Recognizes HCV NS5B from HCV genotype 1 (epitope mapped to NS5B aa 139-392). Does not react with genotype 2. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
Product References
- Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase: J. Schmidt-Mende, et al.; J. Biol. Chem. 276, 44052 (2001)
- Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002)
- Protein-protein interactions between hepatitis C virus nonstructural proteins: Dimitrova, et al.; J. Virol. 77, 5401 (2003)
- Interference of hepatitis C virus RNA replication by short interfering RNAs: Kapadia, et al.; PNAS 100, 2014 (2003)
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