anti-Adiponectin (mouse), mAb (MADI 04)

ELISA: (direct and indirect: 1:5’000-1:10’000)
Immunoprecipitation: (1-5μl per sample in a final reaction volume of 500-1’000μl)
Western blot: (1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Recognizes mouse adiponectin. Detects a band of ~30kDa by Western blot. Weakly cross-reacts with human and rat adiponectin.

Rat IgG1 Isotype Control