Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
anti-RIG-I, mAb (Alme-1)
As low as
495
CHF
CHF 495.00
In stock
Only %1 left
AG-20B-0009-C100100 µgCHF 495.00
Figure 1: Western blot analysis of human RIG-I in HeLa cells by using RIG-I, mAb (Alme-1) (Prod. No. AG-20B-0009).
Method: Cell extracts from HeLa cells either unstimulated (lane 1) or stimulated for 6h (lane 2), 16h (lane 3) or 24 h (lane 4) with Interferon γ were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the RIG-I , mAb (Alme-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system.
Method: Cell extracts from HeLa cells either unstimulated (lane 1) or stimulated for 6h (lane 2), 16h (lane 3) or 24 h (lane 4) with Interferon γ were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the RIG-I , mAb (Alme-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system.
Figure 2: Immunohistochemical staining of endogenous human RIG-I in different human tissues (paraffin sections) using anti-RIG-I, mAb (Alme-1) (Prod. No. AG-20B-0009).
Different human normal tissues (A: Nasopharynx; B: Colon; C: Endometrium) or cancer tissues (D: Breast; E: Skin; F: Ovarian) were stained with anti-RIG-I, mAb (Alme-1) by standard immunohistochemistry.
Different human normal tissues (A: Nasopharynx; B: Colon; C: Endometrium) or cancer tissues (D: Breast; E: Skin; F: Ovarian) were stained with anti-RIG-I, mAb (Alme-1) by standard immunohistochemistry.
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | RIG-1; Retinoic Acid-inducible Gene 1 Protein; DEAD-box Protein 58; Probable ATP-dependent RNA Helicase DDX58 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Alme-1 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant human RIG-I (aa 201-713). |
| Application |
Immunohistochemistry: (paraffin sections) |
| Crossreactivity |
Human Mouse |
| Specificity |
Recognizes human and mouse RIG-I. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-I recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
Product-specific References
- The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008) [PMID: 18756281]
- HIV-1 Vpu neutralizes the antiviral factor Tetherin/BST-2 by binding it and directing its beta-TrCP2-dependent degradation: B. Mangeat, et al.; PLoS Pathog. 5, e1000574 (2009) [PMC2729927]
- Phosphorylation-mediated negative regulation of RIG-I antiviral activity: M.U. Gack, et al.; J. Virol. 84, 3220 (2010) [PMC2838087]
- Mitochondrial dynamics regulate the RIG-I-like receptor antiviral pathway: C. Castanier, et al.; EMBO Rep. 11, 133 (2010) [PMC2828750]
- Molecular mechanism of signal perception and integration by the innate immune sensor retinoic acid-inducible gene-I (RIG-I): M. Binder, et al.; J. Biol. Chem. 286, 27278 (2011) [PMC3149321]
- Incoming RNA Virus Nucleocapsids Containing a 5'-Triphosphorylated Genome Activate RIG-I and Antiviral Signaling: M. Weber, et al.; Cell Host Microbe 13, 336 (2013) [PMC5515363]
- A Distinct Role of Riplet-Mediated K63-Linked Polyubiquitination of the RIG-I Repressor Domain in Human Antiviral Innate Immune Responses: H. Oshiumi, et al.; PLoS Pathog. 9, e1003533 (2013) [PMC3738492]
- Lymphocytic choriomeningitis virus differentially affects the virus-induced type I interferon response and mitochondrial apoptosis mediated by RIG-I/MAVS: C. Pythoud, et al.; J. Virol. 89, 6240 (2015) [PMC4474305]
- ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA: C. Lässig, et al.; eLife 4, e10859 (2015) [PMC4733034]
- A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity: Y.K. Chan & M.U. Gack; Nat. Immunol. 17, 523 (2016) [PMC4837045]
- Sensing of latent EBV infection through exosomal transfer of 5'pppRNA: S.R. Baglio, et al.; PNAS 113, E587 (2016) [PMC4747727]
- RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling: A. Fiegen Durbin, et al.; mBio 7, e00833-16 (2016) [PMC5030355]
- Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation: S. Soonthornvacharin, et al.; Nat. Microbiol. 2, 17022 (2017) [PMC5338947]
- Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity: J.J. Chiang, et al.; Nat. Immunol. 19, 53 (2018) [PMC5815369]
- RIG-I recognizes the 5′ region of dengue and zika virus genomes: M. Chazal, et al.; Cell Rep. 24, 320 (2018) [PMID: 29996094]
- The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling: C. Chiang, et al.; J. Virol. 92, e01737-17 (2018) [PMC5827370; KO Validation]
- Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling: J. Hertzog, et al.; Eur. J. Immunol. 48, 1120 (2018) [PMC6055886]
- Mitochondrial double-stranded RNA triggers antiviral signalling in humans: A. Dhir, et al.; Nature 560, 238 (2018) [PMC6570621]
- Zika virus NS3 mimics a cellular 14-3-3-binding motif to antagonize RIG-I- and MDA5-mediated innate immunity: W. Riedl, et al.; Cell Host Microbe 26, 493 (2019) [PMC6922055]
- Influenza A virus M2 protein triggers mitochondrial DNA-mediated antiviral immune responses: M. Moriyama, et al.; Nat. Commun. 10, 4624 (2019) [PMC6789137]
- Attenuation of the Innate Immune Response against Viral Infection Due to ZNF598-Promoted Binding of FAT10 to RIG-I: G. Wang, et al.; Cell Rep. 28, 1961 (2019) [PMID: 31433974]
- RIG-I-like receptor activation drives type I IFN and antiviral signaling to limit Hantaan orthohantavirus replication: A.M. Kell, et al.; PLoS Pathog. 16, e1008483 (2020) [PMC7202661]
- A ribosomal RNA fragment with 2',3'-cyclic phosphate and GTP-binding activity acts as RIG-I ligand: S. Jung, et al.; Nucleic Acids Res. 48, 10397 (2020) [PMC7544222]
- ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity: G.Q. Liu, et al.; Nat. Microbiol. 6, 467 (2021) [PMC8103894]
- NSs of the mildly virulent sandfly fever Sicilian virus is unable to inhibit interferon signaling and upregulation of interferon-stimulated genes: J.D. Wuerth & F. Weber; J. Gen. Virol. 102, 001676 (2021) [PMC8742993]
- Identifying enhancers of innate immune signaling as broad-spectrum antivirals active against emerging viruses: G. Maarifi, et al.; Cell Chem. Biol. 29, 1113 (2022) [PMC9213012]
- Actin cytoskeleton remodeling primes RIG-I-like receptor activation: D. Acharya, et al.; Cell 185, 3588 (2022) [PMC9680832]
- Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon: A.Y. Lu, et al.; J. Virol. 97, e0198222 (2023) [PMC10231147]
- Acetylation of the NS3 helicase by KAT5γ is essential for flavivirus replication: T. Serman, et al.; Cell Host Microbe 31, 1317 (2023) [PMC10782998]
- K63-linked polyubiquitination of LGP2 by Riplet regulates RIG-I-dependent innate immune response: T. Kouwaki, et al.; EMBO Rep. 24, e54844 (2023) [PMC9900346]
- Differential Cellular Sensing of Fusion from within and Fusion from without during Virus Infection: D.N. Hare, et al.; Viruses 15, 301 (2023) [PMC9962872]
- Cell-type-specific need of Ddx3 and PACT for interferon induction by RNA viruses: N. Sharma, P. Kessler & G.C. Sen; J. Virol. 97, e0130423 (2023) [PMC10734550]
- Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus: E. Genoyer, et al.; Mol. Cell 85, 1147 (2025) [PMC11931551]
- Argonaute 2 inhibits RIG-I signaling via competition for viral RNA binding: H. Liu, et al.; iScience 28, 113391 (2025) [PMC12424427]
- Nef stabilizes actin to prevent HIV-1 sensing by RIG-I-like receptors: A. Laliberté, et al.; Nat. Commun. 16, 10945 (2025) [PMC12686415]
- The inflammasome next door: characterizing pyroptosis induction in HCV-infected and uninfected bystander cells in vitro: H.L. Wallace, et al.; Front. Cell. Infect. Microbiol. 15, 1603739 (2026) [PMC12960644]
Related Products
-
anti-RIG-I, mAb (Alme-1) (Biotin) AG-20B-0009B