anti-Periostin, mAb (Stiny-1)

CHF 470.00
In stock
AG-20B-0033-C100100 µgCHF 470.00
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Product Details
Synonyms Osteoblast-specific Factor 2; OSF-2; Postn
Product Type Monoclonal Antibody
Properties
Clone Stiny-1
Isotype Mouse IgG1κ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Full-length human periostin.
Application

ELISA
Immunohistochemistry: (1:200 - 1:500; paraffin sections and frozen sections)
Western Blot: (1:1'000)
For Functional Application use: anti-Periostin, mAb (blocking) (OC-20) (AG-20B-6000PF)

Crossreactivity Human
Mouse
Specificity

Recognizes human and mouse periostin.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Periostin is a 90-kDa matricellular protein that consists of a typical signal sequence, followed by a cysteine-rich region, an EMI domain (protein-protein interactions), four tandem fasciclin-like domains that are responsible for integrin binding, and a C-terminal region. Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and in osteoblast recruitment, attachment and spreading. Periostin is also involved in many fundamental biological processes such as cell proliferation, cell invasion and angiogenesis. Periostin expression is increased by both transforming growth factor β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2). Changes in periostin expression are commonly detected in various cancers and pre-cancerous conditions, and periostin may be involved in regulating cancer cell activities that contribute to tumorigenesis, cancer progression and metastasis. Periostin has shown to be involved in many aspects of allergic inflammation, such as eosinophil recruitment, airway remodeling, development of a Th2 phenotype and increased expression of inflammatory mediators. It is evaluated as a biomarker for bronchial asthma and airway inflammation.

Product References
  1. Interactions between cancer stem cells and their niche govern metastatic colonization: I. Malanchi, et al.; Nature 481, 85 (2012)
  2. Periostin contributes to the acquisition of multipotent stem cell-like properties in human mammary epithelial cells and breast cancer cells: X. Wang, et al.; PLoS One 8, e72962 (2013)
  3. The perivascular niche regulates breast tumour dormancy: C.M. Ghajar, et al.; Nat. Cell Biol. 15, 807-817 (2013)
  4. Matricellular protein periostin contributes to hepatic inflammation and fibrosis: Y. Huang, et al.; Am. J. Pathol. 185, 786 (2015)
  5. Endogenous semaphorin-7A impedes human lung fibroblast differentiation: S. Esnault, et al.; PLoS One 12, e0170207 (2017)
  6. Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer: Y. Nakazawa, et al., Sci. Rep. 8, (2018)
  7. Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells: Z. Ma, et al.; Cell Rep. 26, 1533 (2019)
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