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AdipoGen Life Sciences
anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free)
290
CHF
CHF 290.00
In stock
AG-20B-6001YPF-C100100 µgCHF 290.00
AG-20B-6001YPF-C500500 µgCHF 730.00
Replaces AG-20B-6001PF
Figure 1: Immunohistochemical analysis on paraffin-embedded sections of human melanoma SK-MEL-28 using anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free) (AG-20B-6001YPF).
Figure 2: Immunocytochemical analysis of human esophageal epithelial cells using anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free) (AG-20B-6001YPF). An anti-mouse IgG secondary antibody was used. Nuclei were counterstained with propidium iodide.
Figure 3: Western blot analysis of human dermal fibroblast whole cell lysate, untreated (left) and treated with TGF-β1 (right) using anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free) (AG-20B-6001YPF). Lysates/proteins at 10 µg per lane. A goat anti-mouse HRP-conjugated monoclonal secondary antibody was used.
| Product Details | |
|---|---|
| Synonyms | FN; Cold-insoluble Globulin; CIG; Fibronectin (EIIIA) |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | IST-9 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant Extra Domain A (EDA) region of human cellular fibronectin. |
| Label/Conjugates | Preservative Free |
| Application |
ELISA |
| Crossreactivity |
Chicken Cow Dog Human Monkey Mouse Pig Rat |
| Specificity |
Recognizes an epitope located in the EDA sequence of human cellular fibronectin (FN). Does not detect extracellular FN (plasma FN). Cross-reacts with mouse, rat, monkey, pig, dog, cow and chicken fibronectin EDA (according to literature). |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Epitope affinity purifed on recombinant fragment of fibronectin EDA. |
| Concentration | Lot dependent |
| Formulation | Sterile liquid. In PBS. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Fibronectin is a major component of the extracellular matrix that binds to integrins, cell surfaces and various compounds including collagen, fibrin, heparin, DNA and actin. It occurs in two main forms: plasma and cellular fibronectin. EDA (Extra Domain A)-containing cellular fibronectin (EDA-FN) plays a major role in cell adhesion, growth, migration and differentiation and it is important for processes such as wound healing and embryonic development. It is associated with fibroblast differentiation by increasing α-SMA expression, collagen deposition, cell contractility and focal adhesion kinase (FAK) activation. EDA-FN promotes cell spread by interacting with the integrins α5-β1, α4-β1 and α9-β1 and has been shown to promote angiogenesis, lymphangiogenesis and metastasis of colorectal tumors. In transformed or tumor-derived cells the EDA segment is about 10-times higher than in fibronectin from normal fibroblasts and it may therefore be a significant marker for malignancy.
Product References
- Monoclonal antibodies in the analysis of fibronectin isoforms generated by alternative splicing of mRNA precursors in normal and transformed human cells: L. Borsi, et al.; J. Cell Biol. 104, 595 (1987)
- Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli: B. Carnemolla, et al.; FEBS Lett. 215, 269 (1987)
- Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma: N. Burton-Wurster & G. Lust; Arch. Biochem. Biophys. 269, 32 (1989)
- Coordinate oncodevelopmental modulation of alternative splicing of fibronectin pre-messenger RNA at ED-A, ED-B, and CS1 regions in human liver tumors: F. Oyama, et al.; Cancer Res. 53, 2005 (1993)
- Endogenous fibronectin of blood polymorphonuclear leukocytes: immunochemical characterization and subcellular localization: R. Salcedo, et al.; Exp. Cell Res. 233, 25 (1997)
- The fibronectin domain ED-A is crucial for myofibroblastic phenotype induction by transforming growth factor-beta1: G. Serini, et al.; J. Cell Biol. 142, 873 (1998)
- Identification of two amino acids within the EIIIA (ED-A) segment of fibronectin constituting the epitope for two function-blocking monoclonal antibodies: Y.F. Liao, et al.; J. Biol. Chem. 274, 17876 (1999)
- Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro: J. Meerschaert, et al.; J. Immunol. 163, 6217 (1999)
- Segmental antigen challenge increases fibronectin in bronchoalveolar lavage fluid: J. Meerschaert, et al.; Am. J. Respir. Crit. Care Med. 159, 619 (1999)
- Deletion of the alternatively spliced fibronectin EIIIA domain in mice reduces atherosclerosis: M.H. Tan, et al.; Blood 104, 11 (2004)
- Fibronectin-alpha4beta1 integrin interactions regulate metalloproteinase-9 expression in steatotic liver ischemia and reperfusion injury: C. Moore, et al.; Am. J. Pathol. 170, 567 (2007)
- Identification of the peptide sequences within the EIIIA (EDA) segment of fibronectin that mediate integrin alpha9beta1-dependent cellular activities: A.V. Shinde, et al.; J. Biol. Chem. 283, 2858 (2008)
- Fibronectin extra domain A (EDA) sustains CD133+/CD44+ subpopulation of colorectal cancer cells: J. Ou, et al.; Stem Cell Res. 11, 820 (2013)
- Irigenin, a novel lead from Western Himalayan chemiome inhibits Fibronectin-Extra Domain A induced metastasis in lung cancer cells: A. Amin, et al.; Sci. Rep. 6, 37151 (2016)
- Detection of soluble ED-A+ fibronectin and evaluation as novel serum biomarker for cardiac tissue remodeling: B. Ziffels, et al.; Dis. Mark. 2016, ID3695454 (2016)
- Fibronectin Extra Domains tune cellular responses and confer topographically distinct features to fibril networks: G. Efthymiou, et al.; J. Cell Sci. 134, jcs252957 (2021)
- Cardiac inducing colonies halt fibroblast activation and induce cardiac/endothelial cells to move and expand via paracrine signaling: S. Mahapatra, et al.; Mol. Biol. Cell 33, ar96 (2022)
- Microcurrent-Mediated Modulation of Myofibroblasts for Cardiac Repair and Regeneration: D.B. Somesh, et al.; Int. J. Mol. Sci. 25, 3268 (2024)
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