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AdipoGen Life Sciences
anti-Isocitrate Dehydrogenase 1 [IDH1] (R132H Mutant) (human), mAb (AG-IHC132)
200
CHF
CHF 200.00
In stock
AG-20B-6024-R100100 µlCHF 200.00
Figure 1: Immunohistochemical staining of mutated IDH1 (R132H) using anti-Isocitrate Dehydrogenase 1 [IDH1] (R132H) (human), mAb (AG-IHC132) (Prod. No. AG-20B-6024) in formalin-fixed and paraffin-embedded (FFPE) human astrocytoma tissue.
| Product Details | |
|---|---|
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | AG-IHC132 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Synthetic IDH1 peptide. |
| Application |
Immunohistochemistry: 1:100 - 1:400 for formalin-fixed, paraffin-embedded tissue sections (frozen sections not validated). Recommended Positive Control: Astrocytoma |
| Crossreactivity | Human |
| Specificity |
Recognizes human IDH1 mutated at R132H. |
| Purity | Protein A/G purified. |
| Formulation | Liquid. In Tris Buffer, pH 7.3 - 7.7, containing 1% BSA and <0.1% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
UniProt Link O75874: IDH1 (human) |
| Accession Number | O75874 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Do not freeze. |
| Use/Stability | Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytosolic enzyme involved in the TCA metabolic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumors, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.
This antibody is intended to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. It has been optimized and validated using the BOND-MAX fully automated IHC&ISH stainer (see Protocol).
Product References
- Consequences of IDH1/2 Mutations in Gliomas and an Assessment of Inhibitors Targeting Mutated IDH Proteins: B. Kaminska, et al., Molecules 24, E968 (2019) (Review)
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