AdipoGen Life Sciences

anti-NMNAT2 (human), pAb

CHF 190.00
In stock
AG-25A-0113-C100100 µgCHF 190.00
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Product Details
Synonyms Nicotinamide Mononucleotide Adenylyltransferase 2; NMN Adenylyltransferase 2; NaMN Adenylyltransferase 2
Product Type Polyclonal Antibody
Properties
Source/Host Guinea pig
Immunogen/Antigen Recombinant human NMNAT2.
Application

ELISA: (direct and indirect: 1:2’000-1:5’000)
Immunohistochemistry: (15μg/ml)
Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.

Crossreactivity Human
Specificity

Recognizes human NMNAT2. Detects a band of ~35kDa by Western blot. Not tested in other species.

Purity Detail Protein A-affinity purified.
Concentration 1 mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

NMNAT2 catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form nicotinic acid mononucleotide (NaMN) as a substrate but with lower efficiency. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans. Has been shown to correlate with Alzheimer’s disease in APPswe/PS1dE9 transgenic mice and delayed wallerian degeneration in cultured superior cervical ganglia (SCGs) from morphological changes, microtubule destruction and neurofilament degradation.

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