anti-α-Actinin-4, pAb (IG-701)

CHF 560.00
In stock
AG-25T-0107-C05050 µgCHF 560.00
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Product Details
Synonyms F-actin Cross-linking Protein
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Synthetic peptide corresponding to aa 27-38 of the N-terminal α-Actinin-4, coupled via a C-terminal cysteine residue to KLH.
Application Immunocytochemistry: (1:250- 1:1'600)
Immunohistochemistry: (1:250- 1:1'600)
Immunoprecipitation: (1:10)
Western Blot: (1:1'000-1:2'500)
Optimal conditions must be determined individually for each application.
Crossreactivity Hamster
Human
Mouse
Pig
Rat
Specificity Recognizes human, mouse, rat, pig and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3.
Purity Antigen affinity purified.
Concentration 250μg/ml
Formulation Liquid. In PBS containing 1mg/ml BSA and 0.01% sodium azide.
Other Product Data Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500 μg supplied at 5mg/ml in SDS sample buffer. Use 5μl (25μg) per lane for Western blotting.
Accession Number UniProt ID O43707: alpha-Actinin-4 (human)
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

α-Actinin 4 is an actin-bundling protein of ~100kDa that is associated with cell motility, endocytosis and cancer invasion. The α-actinin family comprises two non-muscle isoforms (α-actinin-1 and -4) and two skeletal muscle isoforms (α-actinin-2 and -3), with α-actinin-2 being also expressed in cardiac muscle. While α-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the α-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the α-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. α-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, α-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of α-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).

Product References
  1. Expression of α-actinin-4 in acquired human nephrotic syndrome: a quantitative immunoelectron microscopy study: N.P. Goode, et al.; Nephrol. Dial. Transplant.  19, 844 (2004)
  2. The Relationship between Subcellular Localization of Actinin-4 and Cell Motility in Oral Squamous Cell Carcinoma: Y. Iida & H. Chiba; Oral Sci. Intern. 1, 30 (2004)
  3. Parietal Podocytes in Normal Human Glomeruli: J. Beriety, et al.; JASN 17, 2770 (2006)
  4. VASP-dependent regulation of actin cytoskeleton rigidity, cell adhesion, and detachment: A.B. Galler, et al.; Histochem. Cell Biol. 125, 457 (2006)
  5. Extra-cellular matrix proteins induce re-distribution of alpha-actinin-1 and alpha-actinin-4 in A431 cells: A. Bolshakova, et al.; Cell Biol. Int. 31, 360 (2007)
  6. Alpha-actinin is required for tightly regulated remodeling of the actin cortical network during cytokinesis: S. Mukhina, et al.; Dev. Cell 13, 554 (2007)
  7. Expression of alpha-actinin-4 in human diabetic nephropathy: M. Kimura, et al.; Intern. Med. 47, 1099 (2008)
  8. Expression of α-Actinin-1 in Human Glomerular Mesangial Cells In Vivo and In Vitro: C. Yang & W.F. Glass;  Exp. Biol. Med. 233, 689 (2008)
  9. Analysis of Nuclear Protein Complexes Comprising a-Actinin-4 by 2D-Electrophoresis and Mass Spectrometry: M. G. Khotin, et al.; Cell and Tissue Biology 3, 431 (2009)
  10. Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice: B. Hartleben, et al.;  J. Clin. Invest. 120, 1084 (2010)
  11. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4: M. Kumeta, et al.; J. Cell Sci. 123, 1020 (2010)
  12. Albuminuria and Glomerular Damage in Mice Lacking the Metabotropic Glutamate Receptor 1: A. Puliti, et al.; Am. J. Pathol. 178, 1257 (2011)
  13. Nephrotic Syndrome and Subepithelial Deposits in a Mouse Model of Immune-Mediated Anti-Podocyte Glomerulonephritis: C. Meyer-Schwesinger, et al.; J. Immunol. 187, 3218 (2011)
  14. Ubiquitin C-terminal hydrolase-l1 activity induces polyubiquitin accumulation in podocytes and increases proteinuria in rat membranous nephropathy: C. Meyer-Schwesinger, et al.; Am. J. Pathol. 178, 2044 (2011)
  15. Calcium sensitivity of α-actinin is required for equatorial actin assembly during cytokinesis: R. Jayadev, et al.; Cell Cycle 11, 1929 (2012)
  16. Interaction of G-protein βγ complex with chromatin modulates GPCR-dependent gene regulation: A. Bhatnagar, et al.; PLoS ONE 8, e52689 (2013)
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