anti-RasGRP2 (phosphorylated) (pSer587), pAb (IG-1209)
AG-25T-0119-C02020 µgCHF 670.00
Western blot: Human (5μg protein/lane) and mouse (8μg protein/lane) platelets were either not stimulated (-) or were incubated with 5μM Forskolin for 1 min (+). Western blots at the indicated dilutions were performed using anti-RasGRP2 (phosphorylated) (pSer587), pAb (IG-1209) (AG-25T-0119) or anti-RasGRP2, pAb (IG-09) (AG-25T-0118) (loading control), respectively. Both antibodies were incubated in TBS/Tween (0.1%) with 5% or 0.5% milk, respectively.
|Synonyms||CalDAG-GEFI; Ras Guanyl Nucleotide-releasing Protein 2; Ras Guanyl Releasing Protein 2; CDC25-like Protein; CDC25L; Calcium and DAG-regulated Guanine Nucleotide Exchange Factor I; MCG7|
|Product Type||Polyclonal Antibody|
|Immunogen/Antigen||Synthetic phosphopeptide corresponding to a partial sequence of human RasGRP2 containing the phosphorylated serine [pS] of the following sequence stretch (-SFSLPRPGRRG[pS]RPPE-IREEEVQTV-).|
Western Blot: (1:2'000; 100ng/ml)
Optimal conditions must be determined individually for each application.
|Specificity||Recognizes human and mouse RasGRP2 phosphorylated at pSer587 (human isoform 1), pSer586 (mouse) or pSer649 (human isoform 2).|
|Purity Detail||Affinity purified on the phospho-peptide and depleted several-fold on the dephospho-peptide.|
|Formulation||Liquid. In PBS containing 2mg/ml BSA and 0.05% sodium azide.|
|Other Product Data||UniProt ID Q7LDG7: RasGRP2 (human)|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||+4°C|
Do not freeze.
|Use/Stability||Stable for at least 6 months after receipt when stored at +4°C.|
|Product Specification Sheet|
RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.
- Phosphorylation of CalDAG-GEFI by protein kinase A prevents Rap1b activation: H. Subramanian, et. al.; Thromb. Haemost. 11, 1574 (2013)