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AdipoGen Life Sciences
IL-33 (mouse) (rec.) (His)
155
CHF
CHF 155.00
In stock
AG-40A-0053-C01010 µgCHF 155.00
AG-40A-0053-C05050 µgCHF 470.00
Figure 1: Activation of a human ST2-dependent NF-κB pathway.
HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-κB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40hr after transfection, cells were treated with IL33 (mouse) (rec.) (His) (Prod. No. AG-40A-0053) at the concentration as indicated for 8hr, followed by measurement with a dual luciferase assay.
HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-κB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40hr after transfection, cells were treated with IL33 (mouse) (rec.) (His) (Prod. No. AG-40A-0053) at the concentration as indicated for 8hr, followed by measurement with a dual luciferase assay.
| Product Details | |
|---|---|
| Synonyms | Interleukin-33; IL-1F11; NF-HEV |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag. |
| Crossreactivity |
Human Mouse |
| Specificity | Binds to mouse and human ST2. |
| Biological Activity | Activates mouse and human ST2-dependent NF-κB pathway. |
| MW | ~20kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <1EU/μg purified protein (LAL test; Lonza). |
| Formulation | Liquid. 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl. |
| Other Product Data | UniProt link Q2YEJ4: Interleukin-33 (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 2 years after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
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