AdipoGen Life Sciences

CD137L, Soluble (human) (rec.)

CHF 420.00
In stock
AG-40A-0198T-C05050 µgCHF 420.00
More Information
Product Details
Synonyms 4-1BBL; TNFSF9
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

The extracellular domain of human CD137L (aa 50-254) is fused at the N-terminus to a FLAG®-tag.

Crossreactivity Human
Specificity

Binds to human CD137.

MW ~23kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.06EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 50μl sterile water.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS, pH 7.2.
Other Product Data

Uniprot link P41273: CD137L (human)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

The CD137 ligand (CD137L; 4-1BBL) is a member of the tumor necrosis factor (TNF) family. CD137L is a type II transmembrane protein found on activated macrophages, dendritic cells and mature B cells. The interaction with its receptor 4-1BB induces recruitment of TNF receptor-associated factor 1 (TRAF1) and TRAF2 and interaction with the kinase p56lck. CD137 and CD137L have been reported to be involved in tumor rejection, apoptosis, anti-viral immunity, diabetes, in T and B cell co-stimulation and modulation of the immune response.

Product References
  1. Production of recombinant human trimeric CD137L (4-1BBL). Cross-linking is essential to its T cell costimulationactivity: C. Rabu, et al.; J. Biol. Chem. 280, 41472 (2005)
  2. A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade: C. Geuijen, et al.; Nature Commun. 12, 4445 (2021)
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