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AdipoGen Life Sciences
CD137L, Soluble (human) (rec.)
420
CHF
CHF 420.00
In stock
AG-40A-0198T-C05050 µgCHF 420.00
Figure 1: Pull down assay of hCD137L-FLAG (Prod. No. AG-40A-0198T) by hCD137-Fc. Method: 5μg of hCD137-Fc (or control Fc protein), 5μg of hCD137L-FLAG (or control FLAG protein), and protein G resin (or anti-FLAG resin) were incubated in 0.5ml PBS overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hCD137L-FLAG with anti-CD137L (human), mAb (41B436) (Prod. No. AG-20A-0031) or anti-FLAG HRP, respectively.
1. hCD137-Fc + hCD137L-FLAG®-tag
2. hCD137-Fc + hGITRL-FLAG®-tag
3. hCD137-Fc + hGITRL-His-tag
4. hGITR-Fc + hCD137L-FLAG®-tag
5. hGITR-Fc + hGITRL-FLAG®-tag
1. hCD137-Fc + hCD137L-FLAG®-tag
2. hCD137-Fc + hGITRL-FLAG®-tag
3. hCD137-Fc + hGITRL-His-tag
4. hGITR-Fc + hCD137L-FLAG®-tag
5. hGITR-Fc + hGITRL-FLAG®-tag
Figure 2: CD137 (human):Fc (mouse) (rec.) (CHI-HF-211CD137-C100) binds to its ligand CD137L, Soluble (human) (rec.) (Prod. No. AG-40A-0198T). Method: CD137 (human):Fc (mouse) (rec.) is coated on an ELISA plate at 1μg/ml overnight at room temperature. CD137L, Soluble (human) is added (starting at 8μg/ml with a twofold serial dilution) during one hour and then detected using an anti-FLAG® antibody (HRP). The EC50 is around 15ng/ml.
| Product Details | |
|---|---|
| Synonyms | 4-1BBL; TNFSF9 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
The extracellular domain of human CD137L (aa 50-254) is fused at the N-terminus to a FLAG®-tag. |
| Crossreactivity | Human |
| Specificity |
Binds to human CD137. |
| MW | ~23kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.06EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution |
Reconstitute with 50μl sterile water. Add 1X PBS to the desired protein concentration. |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS, pH 7.2. |
| Other Product Data |
Uniprot link P41273: CD137L (human) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The CD137 ligand (CD137L; 4-1BBL) is a member of the tumor necrosis factor (TNF) family. CD137L is a type II transmembrane protein found on activated macrophages, dendritic cells and mature B cells. The interaction with its receptor 4-1BB induces recruitment of TNF receptor-associated factor 1 (TRAF1) and TRAF2 and interaction with the kinase p56lck. CD137 and CD137L have been reported to be involved in tumor rejection, apoptosis, anti-viral immunity, diabetes, in T and B cell co-stimulation and modulation of the immune response.
Product References
- Production of recombinant human trimeric CD137L (4-1BBL). Cross-linking is essential to its T cell costimulationactivity: C. Rabu, et al.; J. Biol. Chem. 280, 41472 (2005)
- A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade: C. Geuijen, et al.; Nature Commun. 12, 4445 (2021)
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