TNF-α, Soluble (human) (rec.)

CHF 120.00
In stock
AG-40B-0006-C01010 µgCHF 120.00
AG-40B-0006-C05050 µgCHF 280.00
AG-40B-0006-30503 x 50 µgCHF 560.00
More Information
Product Details
Synonyms Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin
Product Type Protein
Properties
Source/Host E. coli
Sequence

A FLAG®-tag is fused at the N-terminus of human TNF-α (aa 85-233).

Crossreactivity Human
Mouse
Specificity

Binds to human and mouse TNF-R1.

Biological Activity

Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).

MW ~17kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/µg purified protein (LAL test; Lonza).
Concentration 10 µg size: 0.1 mg/ml after reconstitution.
50 µg size: 1 mg/ml after reconstitution.
Reconstitution 10 µg size: Reconstitute with 100 µl sterile water.
50 µg size: Reconstitute with 50 µl sterile water.
PBS containing at least 0.1% BSA should be used for further dilutions.
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link P01375: TNF-α (human) (precursor)

FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (MultimericTNF-α (Prod. No. AG-40B-0019). Since the MultimericTNF-α mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or MultimericTNF-α can be used.

Product References
  1. CXCL-8/IL8 Produced by Diffuse Large B-cell Lymphomas Recruits Neutrophils Expressing a Proliferation-Inducing Ligand APRIL: B. Manfroi, et al.; Cancer Res. 77, 1097 (2017)
  2. Harnessing Selectivity and Sensitivity in Electronic Biosensing: A Novel Lab-on-Chip Multigate Organic Transistor: V. Parkula, et al.; Anal. Chem. 92, 9330 (2020)
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