AdipoGen Life Sciences

TNF-α, Soluble (human) (rec.)

As low as CHF 95.00

In stock

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AG-40B-0006-C01010 µgCHF 95.00

AG-40B-0006-C05050 µgCHF 215.00

AG-40B-0006-30503 x 50 µgCHF 430.00

Figure 1: TNF-α, Soluble (human) (rec.)(AG-40B-0074) induces NF-κB dependent genes. Methods: Endothelial cells (HUVEC) are incubated for 1h in the presence of BAY 11-7082 (AG-CR1-0013) and then treated with TNFα (20ng/ml) (red bar) or incubated with TNFα (20ng/ml) (in the presence or absence of TNF-R1 (human):Fc (human) (rec.) (0.5µg/ml) (blue and gray bar, respectively). TNFα-induced genes expression such as E-Selectin or MCP-1 are tested by flow cytometry. Picture courtesy of Giulia Vanoni (Dr. Camilla Jandus Lab, University of Lausanne).

Specifications / Handling

More Information
Product Details
Synonyms	Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin
Product Type	Protein
Properties
Source/Host	E. coli
Sequence	A FLAG^®-tag is fused at the N-terminus of human TNF-α (aa 85-233).
Crossreactivity	Human Mouse
Specificity	Binds to human and mouse TNF-R1.
Biological Activity	Induces cell death at a concentration range of 0.01-10ng/ml (ED₅₀= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
MW	~17kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01EU/µg purified protein (LAL test; Lonza).
Concentration	10 µg size: 0.1 mg/ml after reconstitution. 50 µg size: 1 mg/ml after reconstitution.
Reconstitution	10 µg size: Reconstitute with 100 µl sterile water. 50 µg size: Reconstitute with 50 µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
Formulation	Lyophilized. Contains PBS.
Other Product Data	UniProt link P01375: TNF-α (human) (precursor) FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (MultimericTNF-α (Prod. No. AG-40B-0019). Since the MultimericTNF-α mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or MultimericTNF-α can be used.

Product-specific References

CXCL-8/IL8 Produced by Diffuse Large B-cell Lymphomas Recruits Neutrophils Expressing a Proliferation-Inducing Ligand APRIL: B. Manfroi, et al.; Cancer Res. 77, 1097 (2017)
Harnessing Selectivity and Sensitivity in Electronic Biosensing: A Novel Lab-on-Chip Multigate Organic Transistor: V. Parkula, et al.; Anal. Chem. 92, 9330 (2020)
Targeting adrenergic receptors to mitigate invariant natural killer T cells-induced acute liver injury: MB. Gonzatti, et al.; iScience 26, 107947 (2023)

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