Flagellin (rec.)

CHF 230.00
In stock
AG-40B-0125-C01010 µgCHF 230.00
AG-40B-0125-30103 x 10 µgCHF 480.00
More Information
Product Details
Synonyms FliC
Product Type Protein
Properties
Source/Host E. coli
Sequence

The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.

Crossreactivity Human
Mouse
Specificity

Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).

Biological Activity

Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1µg/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5µg/ml on bone-marrow macrophages in the presence of streptolysin O (SLO, 25µg/ml), a protein that allows delivery of exogenous molecules into the cytosol of living cells.

MW ~54kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link P06179: Flagellin (Salmonella typhimurium)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

Product References
  1. Ezh2 competes with p53 to license lncRNA Neat1 transcription for inflammasome activation: J. Yuan, et al.; Nature Cell Death Diff. ahead of print (2022)
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