Frizzled-5 (human):Fc (human) (rec.)
|Synonyms||FzE5; Fz-5; hFz5|
|Source/Host||HEK 293 cells|
The cysteine-rich domain (CRD) of human Frizzled-5 (aa 27-180) is fused at the C-terminus to the Fc portion of human IgG1.
Binds to human and mouse Wnt3a.
Inhibits Wnt3a activation in a stoechiometric manner.
|Endotoxin Content||<0.01EU/μg purified protein (LAL test).|
|Concentration||1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 50μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Protein Negative Control|
|Other Product Data||
Uni-Prot link Q13467: Frizzled-5 (human)
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Wnts are lipid-modified morphogens that control many processes during development, stem cell maintenance and homeostasis and their aberrant regulation has been linked to diseases in man including diabetes, neurodegeneration and cancer. Three distinct receptor families mediate Wnt signaling: Fzs, the single-pass LRP5/6 co-receptors and the atypical tyrosine kinase receptors Ror2 and Ryk. Frizzled proteins can activate canonical Wnt/β-catenin signaling as well as non-canonical pathways (Wnt/Ca2+ pathways and planar cell polarity). The cysteine-rich domain (CRD) of the frizzled receptors situated on the extracellular part of the receptor is sufficient to bind the Wnt ligands.