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AdipoGen Life Sciences
Frizzled-5 (human):Fc (human) (rec.)
290
CHF
CHF 290.00
In stock
AG-40B-0133-C05050 µgCHF 290.00
AG-40B-0133-30503 x 50 µgCHF 575.00
Figure 1: Figure: Frizzled-5 (human):Fc (human) (rec.) (AG-40B-0133) efficiently inhibits Wnt3a activation. Methods: Inhibition of Wnt3a activation by Frizzled-5 (human):Fc (human) (rec.) was measured by using HEK293 cells transfected with a plasmid containing a luciferase gene under the control of a Wnt dependent promoter. Firefly luciferase activity was measured after incubation for 18h at 37°C with 0.5µg/ml of Wnt3a with or without Frizzled-5 (h):Fc (concentrations indicated in the Figure). As negative control, medium only and 20µg/ml Frizzled-5 (h):Fc only did not show any signals (not shown). (Experiment performed by Alexey Koval, Prof. Katanaev Lab, University of Lausanne)
| Product Details | |
|---|---|
| Synonyms | FzE5; Fz-5; hFz5 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
The cysteine-rich domain (CRD) of human Frizzled-5 (aa 27-180) is fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity |
Human Mouse |
| Specificity |
Binds to human and mouse Wnt3a. |
| Biological Activity |
Inhibits Wnt3a activation in a stoechiometric manner. |
| MW | ~50kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
Uni-Prot link Q13467: Frizzled-5 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Wnts are lipid-modified morphogens that control many processes during development, stem cell maintenance and homeostasis and their aberrant regulation has been linked to diseases in man including diabetes, neurodegeneration and cancer. Three distinct receptor families mediate Wnt signaling: Fzs, the single-pass LRP5/6 co-receptors and the atypical tyrosine kinase receptors Ror2 and Ryk. Frizzled proteins can activate canonical Wnt/β-catenin signaling as well as non-canonical pathways (Wnt/Ca2+ pathways and planar cell polarity). The cysteine-rich domain (CRD) of the frizzled receptors situated on the extracellular part of the receptor is sufficient to bind the Wnt ligands.