Frizzled-5 (human):Fc (human) (rec.)

CHF 290.00
In stock
AG-40B-0133-C05050 µgCHF 290.00
AG-40B-0133-30503 x 50 µgCHF 580.00
More Information
Product Details
Synonyms FzE5; Fz-5; hFz5
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

The cysteine-rich domain (CRD) of human Frizzled-5 (aa 27-180) is fused at the C-terminus to the Fc portion of human IgG1.

Crossreactivity Human
Mouse
Specificity

Binds to human and mouse Wnt3a.

Biological Activity

Inhibits Wnt3a activation in a stoechiometric manner.

MW ~50kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 50μl sterile water.
Formulation Lyophilized. Contains PBS.
Protein Negative Control

Fc (human) IgG1 Control (rec.)

Other Product Data

Uni-Prot link Q13467: Frizzled-5 (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Wnts are lipid-modified morphogens that control many processes during development, stem cell maintenance and homeostasis and their aberrant regulation has been linked to diseases in man including diabetes, neurodegeneration and cancer. Three distinct receptor families mediate Wnt signaling: Fzs, the single-pass LRP5/6 co-receptors and the atypical tyrosine kinase receptors Ror2 and Ryk. Frizzled proteins can activate canonical Wnt/β-catenin signaling as well as non-canonical pathways (Wnt/Ca2+ pathways and planar cell polarity). The cysteine-rich domain (CRD) of the frizzled receptors situated on the extracellular part of the receptor is sufficient to bind the Wnt ligands.

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.