Lipoyl-TRIM21 (human) (rec.) (His)
|Synonyms||E3 Ubiquitin-protein Ligase TRIM21; 52 kDa Ro Protein; Tripartite Motif-containing Protein 21|
Human TRIM21 (aa 1-475) is fused at the N-terminus to a Lipoyl domain (bacterial pyruvate dehydrogenase) and at the C-terminus to a 6xHis-tag.
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Concentration||0.1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 100μl sterile water.|
|Formulation||Lyophilized. Contains 50mM Tris-HCl, pH 8 and 300mM NaCl.|
|Other Product Data||
UniProt link P19474: Trim21 (human)
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
TRIM21 (tripartite motif-containing protein 21) is a cytosolic Fc receptor induced by interferon (IFN). TRIM21 is distinguished from other Fc receptors by showing broad antibody specificity as it can activate its functions upon binding to IgG, IgM as well as IgA while other Fc receptors display more restricted antibody isotype and subtype specificities. TRIM21 also functions as a E3 ligase. During infection, antibodies are delivered efficiently to the cytosol when bound to intracellular pathogens such as viruses and bacteria. The antibody-pathogen complex in the cytosol upon engagement of the protein TRIM21 is ubiquitinylated and degraded by the proteasome machinery. Trim-Away is a method to degrade endogenous proteins based on TRIM21, Fc receptor binding and ubiquitin ligase activity. TRIM21, which recognizes antibody-bound proteins of interest, targets them for degradation by the proteasome. The Lipoyl-TRIM21 (human) (His) (rec.) protein is used in the original papers describing Trim-Away.
- A Method for the Acute and Rapid Degradation of Endogenous Proteins: D. Clift, et al.; Cell 172, 1 (2018)
- Acute and rapid degradation of endogenous proteins by Trim-Away: D. Clift, et al.; Nat. Protoc. 10, 2149 (2018)