Lipoyl-TRIM21 (human) (rec.) (His)

CHF 360.00
In stock
AG-40B-0182-C01010 µgCHF 360.00
AG-40B-0182-30103 x 10 µgCHF 720.00
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Product Details
Synonyms E3 Ubiquitin-protein Ligase TRIM21; 52 kDa Ro Protein; Tripartite Motif-containing Protein 21
Product Type Protein
Properties
Source/Host E. coli
Sequence

Human TRIM21 (aa 1-475) is fused at the N-terminus to a Lipoyl domain (bacterial pyruvate dehydrogenase) and at the C-terminus to a 6xHis-tag. 

Crossreactivity Human
Mouse
MW ~68kDa
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Accession Number P19474
Formulation Lyophilized. Contains 50mM Tris-HCl, pH 8 and 300mM NaCl.
Other Product Data

UniProt link P19474: Trim21 (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

TRIM21 (tripartite motif-containing protein 21) is a cytosolic Fc receptor induced by interferon (IFN). TRIM21 is distinguished from other Fc receptors by showing broad antibody specificity as it can activate its functions upon binding to IgG, IgM as well as IgA while other Fc receptors display more restricted antibody isotype and subtype specificities. TRIM21 also functions as a E3 ligase. During infection, antibodies are delivered efficiently to the cytosol when bound to intracellular pathogens such as viruses and bacteria. The antibody-pathogen complex in the cytosol upon engagement of the protein TRIM21 is ubiquitinylated and degraded by the proteasome machinery.  Trim-Away is a method to degrade endogenous proteins based on TRIM21, Fc receptor binding and ubiquitin ligase activity.  TRIM21, which recognizes antibody-bound proteins of interest, targets them for degradation by the proteasome. The Lipoyl-TRIM21 (human) (His) (rec.) protein is used in the original papers describing Trim-Away. 

Product References
  1. A Method for the Acute and Rapid Degradation of Endogenous Proteins: D. Clift, et al.; Cell 172, 1 (2018)
  2. Acute and rapid degradation of endogenous proteins by Trim-Away: D. Clift, et al.; Nat. Protoc. 10, 2149 (2018)
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