AdipoGen Life Sciences

PARP-1 [ARTD1] (E998K Mutant) (human) (rec.) Control

CHF 190.00
In stock
AG-40T-0015-C01010 µgCHF 190.00
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Product Details
Synonyms Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1
Product Type Protein
Properties
Source/Host Sf21 cells
Sequence

Human full-length inactive mutant E988K of PARP-1 is fused to a HA-tag and a His-tag.

Crossreactivity Human
Application

Control

Biological Activity

0.5% of wild type PARP-1.

Purity ≥95% (SDS-PAGE)
Concentration Lot dependent (0.2-0.5mg/ml)
Formulation Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol.
Other Product Data

UniProt ID P09874: PARP-1 (human)

Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-γ to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E988K mutant) is an inactive form of PARP-1 which can be used as a control compound.

Product References
  1. The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function: P.O. Hassa, et al.; J. Biol. Chem. 276, 45588 (2001)
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