PARG (human) (recombinant protein) (His) (highly active)

Specifications / Handling

More Information
Product Details
Synonyms	Poly(ADP-ribose) Glycohydrolase
Product Type	Protein
Properties
Source/Host	Sf21 cells
Sequence	Human PARG is fused to a His-tag.
Crossreactivity	Human
Biological Activity	~100ng is required for poly(ADP-ribose) degradation assays.
Purity	≥95% (SDS-PAGE)
Concentration	Lot dependent (0.1-1.5mg/ml)
Formulation	Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
Other Product Data	UniProt ID Q86W56: PARG (human)
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-20°C
Long Term Storage	-80°C
Handling Advice	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.

Product References

Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases: F. Rosenthal, et al.; Nat. Struct. Mol. Biol. 20, 502 (2013)
Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose): M. Altmeyer, et al.; Nat. Commun. 6, 8088 (2015)
5-diphosphoribose is an endogenous TRPM2 superagonist: R. Fliegert, et al.; Nat. Chem. Biol. 13, 1036 (2017)
PARP1 catalytic variants reveal branching and chain length-specific functions of poly(ADP-ribose) in cellular physiology and stress response: L. Aberle, et al.; Nucleic Acid Res. 48, 10015 (2020)
The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency: M. Gatti, et al.; Cell Rep. 32, 107985 (2020)

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PARG (human) (rec.) (His) (highly active)