NAD Synthetase (M. tuberculosis) (recombinant protein) (His) (recombinant protein)

Specifications / Handling

More Information
Product Details
Synonyms	NADE; Glutamine-dependent NAD(+) Synthetase; EC 6.3.5.1
Product Type	Protein
Properties
Source/Host	E. coli
Sequence	NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
Biological Activity	~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1µmol NAD per min.
MW	~75.0kDa
Purity	≥98% (SDS-PAGE)
Endotoxin Content	N/A
Concentration	0.5mg/ml (Lot specific)
Formulation	Liquid. In 20mM TRIS-HCl, pH 7.8.
Other Product Data	UniProt link P0A5L6: NAD Synthetase (M. tuberculosis)
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-20°C
Long Term Storage	-80°C
Handling Advice	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD⁺, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source.
Catalytic activity: ATP + deamido-NAD⁺ + L-glutamine + H2O = AMP + diphosphate + NAD⁺ + L-glutamate

Product References

Heterologous expression, purification, and enzymatic activity of Mycobacterium tuberculosis NAD(+) synthetase: M. Bellinzoni, et al.; Protein Expr. Purif. 25, 547 (2002)

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NAD Synthetase (M. tuberculosis) (rec.) (His)