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AdipoGen Life Sciences
Acyl-CoA-binding Protein (human) ELISA Kit
610
CHF
CHF 610.00
In stock
AG-45B-0019-KI0196 wellsCHF 610.00
Figure 1: Secretion of human Acyl-CoA-binding Protein during induction of autophagy is measured by the Acyl-CoA-binding Protein (human) ELISA Kit (Prod. No. AG-45B-0019).
Method: Human Embryonic Kidney (HEK 293) (A) and B lymphoblast SKW 6.4 (B) cell lines are treated for 24 hours with different concentrations of the autophagy inducer Everolimus (Prod. No. AG-CN2-0520). Supernatants are collected, diluted and tested using the Acyl-CoA-binding Protein (human) ELISA Kit (Prod. No. AG-45B-0019).
Method: Human Embryonic Kidney (HEK 293) (A) and B lymphoblast SKW 6.4 (B) cell lines are treated for 24 hours with different concentrations of the autophagy inducer Everolimus (Prod. No. AG-CN2-0520). Supernatants are collected, diluted and tested using the Acyl-CoA-binding Protein (human) ELISA Kit (Prod. No. AG-45B-0019).
Figure 2: Standard curve
| Product Details | |
|---|---|
| Synonyms | ACBP; Diazepam-binding Inhibitor; DBI; Endozepine; EP |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity |
Detects human ACBP in serum, plasma and cell culture supernatant. It does not detect mouse ACBP. |
| Crossreactivity | Human |
| Quantity |
1 x 96 wells |
| Sensitivity | 30pg/ml |
| Range | 0.03125 to 2ng/ml |
| Sample Type |
Cell Culture Supernatant Plasma Serum |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Other Product Data |
UniProt link P07108: ACBP (human) |
| Accession Number | Q9BYF1 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use. |
| Use/Stability | 12 months after the day of manufacturing. See expiry date on ELISA Kit box. |
| Documents | |
| Manual |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Acyl-coenzyme A (CoA)-binding protein (ACBP) is an ubiquitously expressed 86 aa polypeptide that binds medium- and long-chain acyl-CoA esters with very high affinity. It plays a role as an intracellular carrier of acyl-CoA esters and regulates lipid metabolism in the cytoplasm of most cell types. In addition to its function within the cells as acyl-coenzyme A (CoA)-binding protein, ACBP also functions as secreted protein called Diazepam-Binding Inhibitor (DBI) that can interact with the benzodiazepine-binding site of the γ-aminobutyric acid (GABA) type A receptor, GABAAR, and modulate its activity. ACBP is secreted upon induction of autophagy (energy deficiency) in different organisms including mouse and human. ACBP levels correlate with human body mass index (BMI). Increasing ACBP levels in mice triggers lipogenesis, food intake and weight gain and neutralization of ACBP increases lipolysis, reduces food intake post-starvation and causes weight loss in mice.
Obese patients exhibit elevated plasma levels of ACBP, while a reduction in the ACBP mRNA and ACBP plasma protein levels is observed in these patients after an important weight loss. ACBP might be useful for the prevention or treatment of obesity and metabolic syndrome diseases.
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