Asprosin (human) Matched Pair Detection Set
|Synonyms||Fibrillin-1 C-terminal Cleavage Product; FBN1 C Terminal Cleavage Product|
|Application Set||Quantitative ELISA|
Detects human Asprosin in cell culture supernatant.
Contains sufficient materials to run ELISAs on 5 x 96-well plates.
|Range||0.156 to 10ng/ml|
|Sample Type||Cell Culture Supernatant|
1 vial standard protein (1µg) (lyophilized) [STD]
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-20°C|
|Handling Advice||Avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Asprosin is a new fasting-induced protein hormone that targets the liver to increase plasma glucose levels. Asprosin is the C-terminal cleavage product of the protein pro-Fibrillin-1. Asprosin is secreted from white adipose tissue and increases hepatic glucose production by using cAMP as a second messenger, leading to activation of protein Kinase A. Reduction of Asprosin levels protects against metabolic syndrome-associated hyperinsulinism. Asprosin may act as a circulating hunger signal. Indeed, peripherally injected recombinant asprosin can cross the blood-brain barrier and intracerebroventricular (i.c.v.) injection of recombinant asprosin stimulated appetite in wild-type mice, indicating a central mechanism of action. Loss of Asprosin in mice and human leads to decreased fat mass and body weight, and hypophagia. Mice are also completely protected from the development of diet-induced obesity. Asprosin works by stimulating the orexigenic AgRP+ (Agouti related neuropeptide) neurons via a cAMP-dependent pathway and by inhibiting the anorexigenic neurons POMC+ (pro-opiomelanocortin) neurons in a GABA-dependent manner. Mutation in Asprosin in human leads to the pattern of metabolic dysregulation, including partial lipodystrophy, accompanied by reduced plasma insulin. Due to its key role in food regulation, Asprosin function could serve as a potentially unique therapeutic target against obesity, diabetes or metabolic diseases.
- Increased plasma asprosin levels in patients with drug-naive anorexia nervosa: Y. Hu, et al.; Eat Weight Disord. (2020)