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AdipoGen Life Sciences
(R)-FSL-1
320
CHF
CHF 320.00
In stock
AG-CP3-0010-C100100 µgCHF 320.00
| Product Details | |
|---|---|
| Synonyms | Fibroblast-stimulating Lipopeptide; R-Pam2CGDPKHPKSF; (R)-Pam2Cys-Gly-Asp-Pro-Lys-His-Pro-Lys-Ser-Phe-OH |
| Product Type | Chemical |
| Properties | |
| Formula |
C84H140N14O18S |
| MW | 1666.2 |
| CAS | 929905-36-2 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | Absence of detectable protein or DNA contaminants with agonistic TLR activity. |
| Appearance | Colorless powder. |
| Solubility | Soluble in water (10mg/ml). Use a homogeniser or sonicator to prepare a homogenous solution. |
| Reconstitution | Reconstitute in 100μl endotoxin-free water (Prod.No. IAX-900-002) to get a 1mg/ml stock solution. |
| Formulation | Lyophilized. |
| Other Product Data |
Note: FSL-1 stimulation induces a MyD88-dependent signaling cascade leading to the activation of NF-κB and the production of proinflammatory cytokines. FSL-1 exhibits a higher stimulatory activity than MALP-2 when tested for its ability to induce the production of TNF-α in macrophages and NF-κB in TLR2/TLR6 transfected HEK293 cells. Depending on the sensitivity of the in vitro assay, the recommended working concentration for specific stimulation of innate immunity via TLR2/TLR6 heterodimers is 10-100nM. FSL-1 is water soluble in contrast to MALP2. |
| InChi Key | VJJLLNHELGKSNA-QWIIDARVSA-N |
| Smiles | [H][C@@](COC(=O)CCCCCCCCCCCCCCC)(CSC[C@]([H])(N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=CN1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O)OC(=O)CCCCCCCCCCCCCCC |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- R-FSL-1 (R-Pam2CGDPKHPKSF) is a synthetic lipoprotein that represents the N-terminal part of the 44kDa lipoprotein LP44 of Mycoplasma salivarium [1].
- The naturally occurring R-stereoisomer is biologically more active than the S-stereoisomer [4].
- Stimulator of TLR2/TLR6 [2-13].
- Inducer of TNF-α production in macrophages [5].
- Upregulates proinflammatory cytokines [3,5].
- Activator of the proinflammatory transcription factor NF-κB [3].
Product References
- The N-terminal lipopeptide of a 44-kDa membrane-bound lipoprotein of Mycoplasma salivarium is responsible for the expression of intercellular adhesion molecule-1 on the cell surface of normal human gingival fibroblasts: K.I. Shibata, et al.; J. Immunol. 165, 6538 (2000)
- Discrimination of bacterial lipoproteins by Toll-like receptor 6: O. Takeuchi, et al.; Int. Immunol. 13, 933 (2001)
- Relationship between structures and biological activities of mycoplasmal diacylated lipopeptides and their recognition by Toll-Like Receptors 2 and 6: T. Okusawa, et al.; Infect. Immun. 72, 1657 (2004)
- Involvement of suppressors of cytokine signaling in toll-like receptor-mediated block of dendritic cell differentiation: H. Bartz, et al.; Blood 108, 4102 (2006)
- Effects of repeated injections of fibroblast-stimulating lipopeptide-1 on fever, formation of cytokines, and on the responsiveness to endotoxin in guinea-pigs: A. Greis, et al.; Acta Physiol. 197, 35 (2009)
- Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide: S. Riekenberg, et al.; FEBS J. 276, 649 (2009)
- Toll-like receptors and innate immunity: H. Kumar, et al.; BBRC 388, 621 (2009)
- Lipoteichoic Acid Induces Unique Inflammatory Responses when Compared to Other Toll-Like Receptor 2 Ligands: E.M. Long, et al.; PLoS One 4, e5601 (2009)
- A new cell-based innate immune receptor assay for the examination of receptor activity, ligand specificity, signalling pathways and the detection of pyrogens: A. Burger-Kentischer, et al.; J. Immunol. Methods 358, 93 (2010)
- Toll-like receptor 2 (P631H) mutant impairs membrane internalization and is a dominant negative allele: G.E. Etokebe, et al.; Scand. J. Immunol. 71, 369 (2010)
- TLR2 stimulation drives human naive and effector regulatory T cells into a Th17-like phenotype with reduced suppressive function: M.H. Nyirenda, et al.; J. Immunol. 187, 2278 (2011)
- A subclass of acylated anti-inflammatory mediators usurp Toll-like receptor 2 to inhibit neutrophil recruitment through peroxisome proliferator-activated receptor gamma: E.M. Long, et al.; PNAS 108, 16357 (2011)
- Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response: K. Wilhelmsen, et al.; Innate Immun. 18, 602 (2012)
- FcεR1-mediated mast cell reactivity is amplified through prolonged Toll-like receptor-ligand treatment: R. Saluja, et al.; PLoS One 7, e43547 (2012)
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