anti-CD8 (human), mAb (14) (APC)

Figure 1: Binding of anti-CD8/APC to human PBL. Methods: Five x 10^5 ficoll-prepared human peripheral blood leucocytes were washed and pre-incubated with 20µl of 300µg/ml human IgG (to reduce non-specific binding) after which they were incubated 45 min on ice with 80µl of anti-CD8/APC at a 1:50 dilution (10µg/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 10.3% subpopulation of the cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to Isotype Control Mouse IgG1/APC (#ANC-278-060). Binding was blocked when cells were pre-incubated 10 min with 20µl of 0.5mg/ml anti-CD8(14) antibody (#ANC-154-020).