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Ancell
anti-CD28 (human), mAb (ANC28.1/5D10)
As low as
371
CHF
CHF 371.00
In stock
Only %1 left
ANC-177-020100 µgCHF 371.00
Specifications / Handling
| Product Details | |
|---|---|
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | ANC28.1/5D10 |
| Isotype | Mouse IgG1κ |
| Immunogen/Antigen | Recombinant Human CD28 and HPB-MLT human tumor cells. |
| Application |
ELISA |
| Crossreactivity | Human |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5mg/ml Gentamicin sulfate |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by Ancell Corporation. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Scientific Background Information
Product Description
Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28 identified as CD80 (B7-1) and CD86 (B7-2) are immunglobulin superfamily monomeric transmembrane glycoproteins of 60kDa and 80kDa respectively. Anti-CD28 antibodies have been shown to have a strong adjuvant effect when conjugated to an immunogen. Monovalency of the CD28 receptor for its natural ligands is essential to provide costimulation without inducing responses in the absense of TCR engagement.
Product-specific References
- Coligation of the Hepatitis C Virus Receptor CD81 with CD28 Primes Naive T Lymphocytes to Acquire Type 2 Effector Function: A. Serra, et al.; J. Immunol. 181, 174 (2008) [PMID: 18566382]
- Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies: Z. Waibler, et al.; PLoS ONE 3, e1708 (2008) [PMC2246163]
- Inhibition of ZAP-70 kinase activity via an analog-sensitive allele blocks T cell receptor and CD28 superagonist signaling: S.E. Levin, et al.; J. Biol. Chem. 283, 15419 (2008) [PMC2397475]
- Selective expansion of memory CD4+ T cells by mitogenic human CD28 generates inflammatory cytokines and regulatory T cells: M. Singh, et al.; Eur. J. Immunol. 38, 1522 (2008) [PMC2735850]
- Granzyme B- and Fas ligand-mediated cytotoxic function induced by mitogenic CD28 stimulation of human memory CD4+ T cells: M.A. Medina, et al.; J. Leukoc. Biol. 91, 759 (2012) [PMC3336770]
- Single CD28 stimulation induces stable and polyclonal expansion of human regulatory T cells: X. He, et al.; Sci. Rep. 7, 43003 (2017) [PMC5320448]
- Immune alterations in subacute sclerosing panencephalitis reflect an incompetent response to eliminate the measles virus: S.P. Yentür, et al.; PLoS ONE 16, e0245077 (2021) [PMC7790413]
- Development of a flow cytometry-based potency assay for prediction of cytokine storms induced by biosimilar monoclonal antibodies: N.H. Madsen, et al.; J. Immunol. Methods 502, 113231 (2022) [PMID: 35122772]
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