anti-CD74 (human), mAb (M-B741) (APC)

Figure 1: Binding of anti-CD74/APC to human Raji cells. Method: Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 µl of anti-CD74/APC at a 1:50 dilution (10 µg/ml). Cells were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.65 log10 fluorescent units when compared to Isoptype Control Mouse IgG2a/APC (#ANC-281-060). Binding was blocked when cells were pre-incubated 10 min with 20 µl of 0.5 mg/ml anti-CD30 antibody (#ANC-179-020).