anti-PPARγ1, 2, mAb (4A4)

PPARγ is a ligand-activated transcription factor involved in the regulation of lipid homeostasis and may function as a master regulator of adipogenesis. PPARγ mRNA is expressed prominently in adipose tissue, but is also found in large intestine, kidney, liver, and small intestine. Alternative splicing of the PPARγ gene results in at least two mRNA species that differ at their 5' ends. Human PPARγ1 and PPARγ2 proteins are 53 and 57 kDa, respectively, based on the deduced amino acid sequences. PPARγ2 is the major PPARγ isoform found in both the cytosolic and nuclear fractions of undifferentiated 3T3-L1 cells. Total cellular PPARγ2 protein increases approximately 2-fold following differentiation of 3T3-L1 cells, whereas only small quantities of PPARγ1 are detected in the nuclear fraction following differentiation.