ENPP1 Fluorescent Inhibitor Screening Assay Kit

Cayman's ENPP1 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human ENPP1, an ectonucleotide pyrophosphatase and phosphodiesterase implicated in multiple biological pathways, including the cGAS/STING pathway, and bone mineralization. The assay uses an ENPP1-specific fluorogenic substrate, Tokyo Green-mAMP (TG-mAMP). ENPP1 cleaves this substrate generating free Tokyo Green, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 485 and 520 nm, respectively. The potent and reversible ENPP1 inhibitor ENPP1 Inhibitor C Assay Reagent is included as a positive control.