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Chemodex
AMCA-H
Product Details | |
---|---|
Synonyms | 7-Amino-4-methyl-3-coumarinylacetic acid |
Product Type | Chemical |
Properties | |
Formula |
C12H11NO4 |
MW | 233.22 |
CAS | 106562-32-7 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥90% (HPLC) |
Appearance | Brown solid. |
Solubility | Soluble in DMSO or DMF. |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | QEQDLKUMPUDNPG-UHFFFAOYSA-N |
Smiles | CC1=C(CC(O)=O)C(=O)OC2=C1C=CC(N)=C2 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
(1) G-L. Ferri et al.; J. Histochem. Cytochem. 45(2), 155 (1997) | (2) B. Ufhake et al.; J. Neurosc. Meth. 40(1), 39 (1991) | (3) M. W. Wessendorf et al.; J. Histochem. Cytochem. 38(1), 87 (1990)