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Chemodex
7-Amino-4-trifluoromethylcoumarin
97
CHF
CHF 97.00
In stock
CDX-A0029-G0011 gCHF 97.00
| Product Details | |
|---|---|
| Synonyms | Coumarin 151; AFC |
| Product Type | Chemical |
| Properties | |
| Formula |
C10H6F3NO2 |
| MW | 229.16 |
| CAS | 53518-15-3 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥99% (GC) |
| Appearance | Yellow solid. |
| Solubility | Soluble in DMSO, DMF or methanol (all 25mg/ml). Insoluble in water. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | JBNOVHJXQSHGRL-UHFFFAOYSA-N |
| Smiles | NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at RT. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
7-Amino-4-trifluoromethylcoumarin (AFC) is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids and therefore the precursor for a variety of AFC enzyme substrates. It is a fluorogenic standard useful as a calibration standard for AFC-based enzyme substrates and for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC. AFC is also suitable as laser dye. Spectral Data: λex 376nm, λem 482nm, Quantum yield 0.531.
Product References
(1) K.H. Drexhage, et al.; Opt. Commun. 15, 399 (1975) | (2) R.E. Smith, et al.; Thromb. Res. 17, 393 (1980) | (3) E.R. Bissell, et al.; J. Org. Chem. 45, 2283 (1980) | (4) R.E. Smith, et al.; Histochem. J. 24, 637 (1992) | (5) G. Nowak; J. Biol. Chem. 277, 43377 (2002) | (6) C.-S. Chu & Y.-L. Lo; Sensors and Actuators B: Chemical. 134, 711 (2008) | (67) V. Stanisic, et al.; J. Bio. Chem. 284, 16135 (2009) | (8) J.-G. Lee, et al.; J. Bio.. Chem. 289, 3510 (2014)