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Chemodex
9-Amino-6-chloro-2-methoxyacridine
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258
CHF
CHF 258.00
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CDX-A0099-M02525 mgCHF 258.00
CDX-A0099-M05050 mgCHF 451.00
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| Product Details | |
|---|---|
| Synonyms | ACMA; 2-Methoxy-6-chloro-9-aminoacridine; 9-Amino-3-chloro-7-methoxyacridine; G 185; NSC 15300 |
| Product Type | Chemical |
| Properties | |
| Formula | C14H11ClN2O |
| MW | 258.7 |
| CAS | 02.09.3548 |
| RTECS | AR7330000 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | Yellow to orange powder. |
| Solubility | Soluble in DMSO, DMF or methanol (1mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | IHHSSHCBRVYGJX-UHFFFAOYSA-N |
| Smiles | ClC1=CC=C(C(N)=C(C=C(OC)C=C2)C2=N3)C3=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
9-Amino-6-chloro-2-methoxyacridine (ACMA) is a cell-permeable ph-sensitive fluorescent probe that intercalates into DNA. It selectively binds to poly(dA-dT) sequences with the fluorescence lifetime decreasing with incorporation of guanosine. It is used for nucleic acid staining/labeling. ACMA fluorescence is pH-dependent and is quenched when a pH gradient is established. Spectral Data: λex 411nm; λem 475nm (in methanol). Excitation of the ACMA-DNA complex (excitation/emission maxima λ419/483 nm) is possible with most UV-light sources, making it compatible for use with both shorter- and longer-wavelength dyes. ACMA also apparently binds to membranes in the energized state and becomes quenched if a pH gradient forms. It has been extensively employed to follow cation and anion movement across membranes and to study the proton-pumping activity of various membrane-bound ATPases. ACMA also inhibits acetylcholinesterase.
Product References
(1) C. Helene, et al.; Biochem. Soc. Trans. 14, 201 (1986) | (2) T. Hard, et al.; J. Phys. Chem. 93, 4338 (1989) | (3) H. Rottenberg & R. Moreno-Sanchez; Biochim. Biophys. Acta 1183, 161 (1993) | (4) S.D. Watts & R.A. Capaldi; J. Biol. Chem. 272, 15065 (1997) | (5) K. Fukui, et al.; J. Photochem. Photobiol. B Biol. 50, 18 (1999) | (6) S. Bencharit, et al.; Chem. Biol. 10, 341 (2003) | (7) I. Carqueijeiro, et al.; Methods Mol. Biol. 1405, 121 (2016) | (8) P. Uzdavinys, et al.; PNAS 114, E1101 (2017)