6-Amino-D-luciferin

CHF 77.00
In stock
CDX-A0306-M0011 mgCHF 77.00
CDX-A0306-M0055 mgCHF 153.00
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Product Details
Synonyms 4,5-Dihydro-2[6-amino-2-benzthiazolyl]-4-thiazole carboxylic acid
Product Type Chemical
Properties
Formula C11H9N3O2S2
MW 279.34
CAS 161055-47-6
Source/Host Chemicals Synthetic.
Purity Chemicals ≥99% (HPLC)
Appearance Yellow powder.
Solubility Soluble in water.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key HKSJKXOOBAVPKR-SSDOTTSWSA-N
Smiles NC1=CC2=C(C=C1)N=C(S2)C1=N[C@H](CS1)C(O)=O
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

Amino analog of the common D-luciferin substrate for use in measuring firefly luciferase activity. This cell permeable reagent can be used when considering design of proluminescent bioconjugates (peptidase substrates) of luciferin for ultrasensitive luminescent assays. In bioluminescent protease assays peptides or amino acids are conjugated to the luciferin analog 6-amino-D-luciferin. These substrates are useful for ultrasensitive analysis of a variety of protease enzymes including various caspases and cathepsins, chymotrypsin, trypsin, elastase, kallikrein, thrombin, ficin, bromoalain, plasmin, papain, ficin and many others or even for use in vivo tracing of luciferase transfected cells in living tissues. The resulting bioluminescence spectrum of reaction of 6-Amino-D-luciferin is very similar to that observed for the natural substrate D-luciferin. Peptidase assays using a bioluminogenic substrate can be easily adapted to 96-well formats for HTS-based assays. Using this principle, it is possible to design an assay to detect almost any peptidase or protease activity via bioluminescence by using a substrate with a suitable peptide sequence. Applications in pathogen detection, discovery of protease inhibitors, probing cell physiology and assessing protease activity in oncogenesis are possible at extraordinary sensitivity.

Product References

(1) R. Shinde, et al.; Biochemistry 45, 11103 (2006) | (2) A. Dragulescu-Andrasi, et al.; Bioconjugate Chem. 20, 1660 (2009) | (3) H. Takakura, et al.; Chem. Asian J. 5, 2053 (2010) | (4) K.R. Harwood, et al.; Chem. Biol. 18, 1649 (2011) | (5) S.J. Orcutt, et al.; BBA Mol. Res. 1823, 2079 (2012) | (6) Z-S. Li, et al.; J. Photochem. Photobiol. A: Chem. 243, 7 (2012) | (7) R. Kojima, et al.; Angew. Chem. Int. Ed. 52, 1175 (2013) | (8) J. Li, et al.; Anal. Chem. 86, 2747 (2014) | (9) V.R. Viviani, et al.; Biochemistry 53, 5208 (2014) | (10) D.M. Mofford, et al.; JACS 136, 13277 (2014) | (11) Y. Nam, et al.; Chem. Commun. 52, 1128 (2016)

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