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Chemodex
BCIP p-toluidine salt
45
CHF
CHF 45.00
In stock
CDX-B0024-M100100 mgCHF 45.00
CDX-B0024-G0011 gCHF 335.00
| Product Details | |
|---|---|
| Synonyms | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; X-phosphate p-toluidine salt |
| Product Type | Chemical |
| Properties | |
| Formula |
C8H6BrClNO4P . C7H9N |
| MW | 433.62 |
| CAS | 6578-06-9 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | Powder. |
| Solubility | Soluble in DMSO (25mg/ml) or DMF (25mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
| Smiles | BrC1=CC=C(NC=C2OP([O-])(O)=O)C2=C1Cl.[NH3+]C3=CC=C(C)C=C3 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).
Product References
(1) E. Epstein, et al.; Am. J. Clin. Pathol. 48, 530 (1967) | (2) P.L. Wolf, et al.; Experientia 23, 183 (1967) | (3) P.L. Wolf, et al.; Enzymologia 35, 154 (1968) | (4) C.-I. Chang, et al.; Sensors 12, 2710 (2012) | (5) Y. Li, et al.; Anal. Sci. 32, 215 (2016)