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Chemodex
6-Carboxy-2',7'-dichlorofluorescein DA
244
CHF
CHF 244.00
In stock
CDX-C0334-M100100 mgCHF 244.00
CDX-C0334-M250250 mgCHF 489.00
| Product Details | |
|---|---|
| Synonyms | 6-CDCF DA; 6-Carboxy-DCF DA |
| Product Type | Chemical |
| Properties | |
| Formula |
C25H14CI2O9 |
| MW | 529.29 |
| CAS | 144489-10-1 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥97% (NMR) |
| Appearance | White to off-white powder. |
| Solubility | Soluble in DMSO or chloroform. Insoluble in water. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | FCNONRRKVDBQRD-UHFFFAOYSA-N |
| Smiles | O=C(O1)C2=C(C=C(C(O)=O)C=C2)C31C4=C(C=C(OC(C)=O)C(Cl)=C4)OC5=CC(OC(C)=O)=C(Cl)C=C53 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Fluorescent indicator. The non-fluorescent 6-CDCF DA passively diffuses across the cell membrane and is hydrolyzed to fluorescent CDCF by intracellular esterases. It has been shown to be highly sensitive, long-lasting, cell-permeant and fluorescein-based tracer for quantifying ROS generation. Substrate for MRP2 and MRP3 and therefore the function of the efflux transporters (hepatic exrection) and multi-drug resistance associated proteins MRP2 and MRP3 can be measured using 6-CDCF DA. The CDCFDA/CDCF technoique provides a fast screening tool for MRP2 substrates and/or inhibitors. Spectral data: λEx=502nm; λEm=530nm.
Product References
(1) J.P. Kehrer & T. Paraidathathu; Free Rad. Res. Commun. 16, 217 (1992) | (2) M.J. Zamek-Gliszczynski, et al.; J. Pharmacol. Exp. Ther. 304, 801 (2003) | (3) S. Siissalo, et al.; Eur. J. Pharm. Biopharm. 71, 332 (2009) | (4) A.I. Pogue, et al.; Int. J. Mol. Sci. 13, 9615 (2012) | (5) J.A.Sinclair, et al.; Toxicol. In Vitro 27, 451 (2013)