2-Chloro-2'-deoxyadenosine

2-Chloro-2′-deoxyadenosine is a synthetic anticancer purine nucleoside analog commonly used in biochemical and cellular research to study nucleoside metabolism, DNA synthesis, and apoptosis pathways. Due to its resistance to adenosine deaminase–mediated degradation, this compound exhibits enhanced stability in biological systems, making it a valuable tool for mechanistic studies involving DNA replication and repair. Cladribine is cytotoxic to resting or proliferating lymphocytes. It inhibits RNA synthesis and induces DNA strand breaks in resting human peripheral blood lymphocytes. Cladribine induces cell cycle arrest at the G1 phase and apoptosis in U266, RPMI-8226, and MM.1S multiple myeloma cells in a concentration-dependent manner. It reduces tumor growth in HT-29 colon cancer, RL lymphoma, and RPMI-8226 multiple myeloma mouse xenograft models.