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Chemodex
2',7'-Dichlorofluorescin diacetate
34
CHF
CHF 34.00
In stock
CDX-D0064-M05050 mgCHF 34.00
CDX-D0064-M250250 mgCHF 108.00
CDX-D0064-G0011 gCHF 272.00
| Product Details | |
|---|---|
| Synonyms | H2DCFDA; 2',7'-Dichlorodihydrofluorescein diacetate; Dichlorofluorescin DA; DCFDA; DCFH; DCFH-DA |
| Product Type | Chemical |
| Properties | |
| Formula |
C24H16Cl2O7 |
| MW | 487.29 |
| CAS | 4091-99-0 |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | White to off-white powder. |
| Solubility | Soluble in DMSO (30mg/ml) or ethanol(5mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | PXEZTIWVRVSYOK-UHFFFAOYSA-N |
| Smiles | ClC1=C(OC(C)=O)C=C2C(C(C3=CC=CC=C3C(O)=O)C(C=C(Cl)C(OC(C)=O)=C4)=C4O2)=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Dichlorofluorescin diacetate (H2DCFDA) is a chemically reduced analog of fluorescein, used as an indicator for reactive oxygen species (ROS) in cells. The cell permeable non-fluorescent H2DCFDA is cleaved by cellular esterases and oxidation, and converted to the highly fluorescent 2',7'-dichlorofluorescein. H2DCFDA might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. This probe is widely used to monitoring cellular redox processes. Used as a component/substrate of the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS). Can detect reactive oxygen species in live cells. Spectral data: λEx=504nm/λEm=529nm.
Product References
(1) A.S. Ferrer, et al.; Anal. Biochem. 187, 129 (1990) | (2) C.P. LeBel, et al.; Toxicol. Appl. Pharmacol. 104, 17 (1990) | (3) A.R. Rosenkranz, et al.; J. Immunol. Meth. 156, 39 (1992) | (4) F. Perez-Garcia, et al.; Life Sci. 59, 2033 (1996) | (5) J.P. Crow; Nitric Oxide 1, 145 (1997) | (6) N.W. Kooy, et al.; Free Rad. Res. 27, 245 (1997) | (7) C. Gabriel, et al.; J. Pharmacol. Toxicol. Meth. 38, 93 (1997) | (8) O. Myhre, et al.; Biochem. Pharmacol. 65, 1575 (2003) | (9) D. Figueroa, et al.; J. Pharmacol. Toxicol. Meth. 94, 26 (2018)