Dihydrorhodamine 123

Specifications / Handling

More Information
Product Details
Synonyms	DHR
Product Type	Chemical
Properties
Formula	C₂₁H₁₈N₂O₃
MW	346.38
CAS	109244-58-8
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥95% (NMR)
Appearance	Pink solid.
Solubility	Soluble in DMSO, DMF or ethanol.
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	FNEZBBILNYNQGC-UHFFFAOYSA-N
Smiles	COC(=O)C1=CC=CC=C1C1C2=CC=C(N)C=C2OC2=C1C=CC(N)=C2
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Cell-permeable non-fluorescent reactive oxygen species (ROS) indicator. Passively diffuses across membranes where it is oxidized by peroxynitrite to rhodamine 123 which localizes in the mitochondria and exhibits green fluorescence (Ex/Em wavelengths of 500 and 536 nm). Used to investigate reactive oxygen intermediates produced by human and murine phagocytes, activated rat mast cells and cultured endothelial cells. In addition, it has been used together with Fura Red calcium indicator to simultaneously measure oxidative bursts and Ca2+ fluxes in monocytes and granulocytes. Dihydrorhodamine 123 has been shown to be a more sensitive probe than H2DCFDA for detecting granulocyte respiratory bursts.

Product References

(1) K. Hensley et al.; Meth. Biol. Oxidative Stress 169 (2003) | (2) H. Ischiropoulos et al.; Meth. Enzymology 301, 367 (1999) | (3) L.M. Henderson et al.; Europ. J. Biochem. FEBS 217(3), 973 (1993) An unappreciated role for neutrophil-DC hybrids in immunity to invasive fungal infections: J.S. Fites, et al.; PLoS Pathog. 14, e1007073 (2018)

The Salmonella type-3 secretion system-1 and flagellar motility influence the neutrophil respiratory burst: T.L. Westerman, et al.; PLoS One 13, e0203698 (2018)

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