Dihydrorhodamine 123

CHF 84.00
In stock
CDX-D0134-M0022 mgCHF 84.00
CDX-D0134-M01010 mgCHF 205.00
CDX-D0134-M125125 mgCHF 1'932.00
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Product Details
Synonyms DHR
Product Type Chemical
Formula C21H18N2O3
MW 346.38
CAS 109244-58-8
Source/Host Chemicals Synthetic.
Purity Chemicals ≥95% (NMR)
Appearance Pink solid.
Solubility Soluble in DMSO, DMF or ethanol.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.

Smiles COC(=O)C1=CC=CC=C1C1C2=CC=C(N)C=C2OC2=C1C=CC(N)=C2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

Cell-permeable non-fluorescent reactive oxygen species (ROS) indicator. Passively diffuses across membranes where it is oxidized by peroxynitrite to rhodamine 123 which localizes in the mitochondria and exhibits green fluorescence (Ex/Em wavelengths of 500 and 536 nm). Used to investigate reactive oxygen intermediates produced by human and murine phagocytes, activated rat mast cells and cultured endothelial cells. In addition, it has been used together with Fura Red calcium indicator to simultaneously measure oxidative bursts and Ca2+ fluxes in monocytes and granulocytes. Dihydrorhodamine 123 has been shown to be a more sensitive probe than H2DCFDA for detecting granulocyte respiratory bursts.

Product References

(1) K. Hensley et al.; Meth. Biol. Oxidative Stress 169 (2003) | (2) H. Ischiropoulos et al.; Meth. Enzymology 301, 367 (1999) | (3) L.M. Henderson et al.; Europ. J. Biochem. FEBS 217(3), 973 (1993) An unappreciated role for neutrophil-DC hybrids in immunity to invasive fungal infections: J.S. Fites, et al.; PLoS Pathog. 14, e1007073 (2018)

The Salmonella type-3 secretion system-1 and flagellar motility influence the neutrophil respiratory burst: T.L. Westerman, et al.; PLoS One 13, e0203698 (2018)

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