CHF 34.00
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CDX-D0156-G0011 gCHF 34.00
CDX-D0156-G0055 gCHF 75.00
CDX-D0156-G02525 gCHF 170.00
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Product Details
Synonyms Ellman's Reagent; 5,5′-Dithiobis(2-nitrobenzoic acid)
Product Type Chemical
Formula C14H8N2O8S2
MW 396.35
CAS 69-78-3
RTECS DG9650000
Source/Host Chemicals Synthetic
Purity Chemicals ≥98% (Titration)
Appearance Yellow powder.
Solubility Soluble in ethanol, dioxane or phosphate buffer.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles OC(=O)C1=CC(SSC2=CC=C(C(=C2)C(O)=O)[N+]([O-])=O)=CC=C1[N+]([O-])=O
Shipping and Handling
Shipping AMBIENT
Short Term Storage +20°C
Long Term Storage +20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.

Product References

(1) G.L. Ellman; Arch. Biochem. Biophys. 82, 70 (1959) | (2) H.B. Collier; Anal. Biochem. 56, 310 (1973) | (3) P.W. Riddles, et al.; Meth. Enzymol. 91, 49 (1983) | (4) C.K. Riener, et al.; Anal. Bioanal. Chem. 373, 266 (2002) | (5) J. Sedlak & R.H. Lindsay; Anal. Biochem. 25, 192 (1968) | (6) G.L. Ellman; Biochem. Pharmacol. 7, 88 (1961) | (7) M.J. Gething & B.E. Davidson; Eur. J. Biochem. 30, 352 (1972) | (8) A.F.S.A. Habeeb; Meth. Enzymol. 25, 457 (1972) | (9) D. Ming, et al.; Biotechniques 18, 808 (1995)

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