Chemodex

Dibenzylfluorescein

CHF 204.00
In stock
CDX-D0282-M100100 mgCHF 204.00
CDX-D0282-M500500 mgCHF 543.00
More Information
Product Details
Synonyms DBF; NSC 645658
Product Type Chemical
Properties
Formula

C34H24O5

MW 512.55
CAS 97744-44-0
Source/Host Chemicals Synthetic
Purity Chemicals ≥98% (NMR)
Appearance Faint to dark orange powder.
Solubility Soluble in DMSO (10mg/ml) or DMF (20mg/ml).
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key YZJGKSLPSGPFEV-UHFFFAOYSA-N
Smiles O=C(OCC1=CC=CC=C1)C2=CC=CC=C2C(C3=CC=C(OCC4=CC=CC=C4)C=C3O5)=C6C5=CC(C=C6)=O
Shipping and Handling
Shipping AMBIENT
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF
Description

Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19 and aromatase (CYP19). It is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM. The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.

Product References

(1) D.M. Stresser, et al.; Drug Metab. Dispos. 28, 1440 (2000) | (2) M.T. Donato, et al.; Drug Metab. Dispos. 32, 699 (2004) | (3) E.R. Troesken, et al.; Toxicol. 219, 33 (2006) | (4) K.A. Salminen, et al.; Drug Metab. Dispos. 39, 412 (2011) | (5) D. Moutinho, et al.; Drug Metab. Dispos. 40, 754 (2012)

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