DAF-2 Solution

CHF 530.00
In stock
CDX-D0584-M0011 mgCHF 530.00
More Information
Product Details
Synonyms 4,5-Diaminofluorescein
Product Type Chemical
Formula C20H14N2O5
MW 362.34
CAS 205391-01-1
Source/Host Chemicals Synthetic.
Purity Chemicals ≥95% (HPLC)
Appearance Liquid.
Solubility Soluble in DMSO.
Concentration 5mM in DMSO (1mg in 0.55ml DMSO)
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells. Spectral data: λex 505nm; λem 518nm in 0.1M Tris pH8.0.

Product References

(1) M. Kirsch et al.; J. Pineal Res. 40(1), 10 (2006) | (2) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003) | (3) N. Nagata et al.; J. Biochem. 125(4), 658 (1999) | (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998) | (5) H. Kojima et al.; Anal. Chem. 70, 2446 (1998)

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