Chemodex

DAPI dihydrochloride Solution (10 mM in water)

CHF 116.00
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CDX-D1217-M0055 mgCHF 116.00
CDX-D1217-M01010 mgCHF 174.00
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Product Details
Synonyms 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; 4',6-Diamidino-2-phenylindole dihydrochloride; FxCycle Violet
Product Type Chemical
Properties
Formula C16H15N5 . 2HCl
MW 350.25
CAS 28718-90-3
Purity Chemicals ≥95% (HPLC)
Appearance Liquid.
Solubility Soluble in water.
Concentration 10mM in water
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles NC(C1=CC=C2C(NC(C3=CC=C(C(N)=N)C=C3)=C2)=C1)=N.Cl.Cl
Shipping and Handling
Shipping AMBIENT
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF
Description

DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that is a minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma. Spectral Data: Excitation: λex 340 nm; Emission: λem 488 nm (only DAPI). Excitation: λex 360nm; Emission: λem 460nm (DAPI-DNA complex).

Product References

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