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Chemodex
3-(2-Furoyl)quinoline-2-carboxaldehyde
Product Details | |
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Synonyms | FQ; FQCA; ATTO-TAG™ FQ Derivatization Reagent |
Product Type | Chemical |
Properties | |
Formula |
C15H9NO3 |
MW | 251.24 |
CAS | 126769-01-5 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥95% (HPLC) |
Appearance | White to off-white powder. |
Solubility | Soluble in methanol. |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | PNCHURHVMDRFTR-UHFFFAOYSA-N |
Smiles | [H]C(=O)C1=NC2=CC=CC=C2C=C1C(=O)C1=CC=CO1 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
3-(2-Furoyl)quinoline-2-carboxaldehyde (FQCA) is a fluorogenic derivatizing reagent for liquid chromatography suitable for high-sensitivity analysis of amino acids by laser-induced fluorescence with an argon-ion laser. The reagent forms highly fluorescent isoindoles upon reaction with primary amines. It is used for the determination of isoelectronic points (pI values) of proteins with laser-induced fluorescence detection (c-IEF-LIF). C-IEF-LIF technique can routinely detect 10-11 M of FQCA-labeled protein, compared with 10-7 M by traditional slab gel IEF and silver staining. Spectral Data: λex=~480nm, λem=~590nm.
(1) D. Pinto et al.; J. Chrom., B 793(1), 107 (2003) | (2) D.P. Richards et al.; J. Chromatog. A. 853, 21 (1999) | (3) I.H. Lee et al.; Anal. Chem. 70, 4546 (1998) | (4) D.B. Craig et al.; Electrophoresis 19, 2175 (1998) | (5) S.C. Beale et al.; J. Chromatog. 499, 579 (1990)