|Synonyms||1'(3'H)-isobenzofuran]-3,3'-dione, 4-phenyl-spiro[furan-2(3H); 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione; Fluram|
|Purity Chemicals||≥98% (TLC)|
|Appearance||Off-white to yellow powder.|
|Solubility||Soluble in acetone (50 mg/ml), water (partly), ethanol, chloroform, DMSO or acetonitrile.|
|Identity||Determined by NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||+20°C|
|Long Term Storage||+20°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at +20°C.|
|Product Specification Sheet|
A non-fluorescent reagent that reacts readily under mild conditions with primary amino groups in amino acids and peptides to form stable and highly fluorescent derivatives. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analysis by the Beckman automated sequencer. Spectral data: Exc. 390nm/Em. 475 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine.
(1) S. Udenfriend, et al.; Science 178, 871 (1972) | (2) M. Weigele, et al.; JACS 94, 5927 (1972) | (3) S. Stein, et al.; Arch. Biochem. Biophys. 155, 202 (1973) | (4) P. Bohlen, et al.; Arch. Biochem. Biophys. 163, 390 (1974) | (5) C.Y. Lai, et al.; Meth. Enzymol. 47, 236 (1977) | (6) K. Sogowa & K.J. Takahaski; Biochem. 83, 1783 (1978) | (7) A.S. Bhown, et al.; Anal. Biochem. 112, 158 (1981)