Fluorescamine

CHF 70.00
In stock
CDX-F0003-M100100 mgCHF 70.00
CDX-F0003-M250250 mgCHF 128.00
CDX-F0003-G0011 gCHF 459.00
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Product Details
Synonyms 1'(3'H)-isobenzofuran]-3,3'-dione, 4-phenyl-spiro[furan-2(3H); 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione; Fluram
Product Type Chemical
Properties
Formula C17H10O4
MW 278.26
CAS 38183-12-9
Source/Host Chemicals Synthetic
Purity Chemicals ≥98% (TLC)
Appearance Off-white to yellow powder.
Solubility Soluble in acetone (50 mg/ml), water (partly), ethanol, chloroform, DMSO or acetonitrile.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key ZFKJVJIDPQDDFY-UHFFFAOYSA-N
Smiles O=C1OC2(OC=C(C2=O)C2=CC=CC=C2)C2=CC=CC=C12
Shipping and Handling
Shipping AMBIENT
Short Term Storage +20°C
Long Term Storage +20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

A non-fluorescent reagent that reacts readily under mild conditions with primary amino groups in amino acids and peptides to form stable and highly fluorescent derivatives. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analysis by the Beckman automated sequencer. Spectral data: Exc. 390nm/Em. 475 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine.

Product References

(1) S. Udenfriend, et al.; Science 178, 871 (1972) | (2) M. Weigele, et al.; JACS 94, 5927 (1972) | (3) S. Stein, et al.; Arch. Biochem. Biophys. 155, 202 (1973) | (4) P. Bohlen, et al.; Arch. Biochem. Biophys. 163, 390 (1974) | (5) C.Y. Lai, et al.; Meth. Enzymol. 47, 236 (1977) | (6) K. Sogowa & K.J. Takahaski; Biochem. 83, 1783 (1978) | (7) A.S. Bhown, et al.; Anal. Biochem. 112, 158 (1981)

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