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Chemodex
5(6)-FITC
Product Details | |
---|---|
Synonyms | Fluorescein 5(6)-isothiocyanate |
Product Type | Chemical |
Properties | |
Formula | C21H11NO5S |
MW | 389.38 |
CAS | 27072-45-3 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥95% (HPLC) |
Appearance | Yellow to orange powder. |
Solubility | Soluble in aqueous buffers (pH > 6). |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | QRQQVJDEAPQYJW-UHFFFAOYSA-N |
Smiles | CN=C=S.OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=C(O2)C=C(O)C=C1 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.
(1) R.W. Dirks et al.; J. Histochem. Cytochem 38, 467 (1990) | (2) S. Wu et al.; J. Chromatogr 480, 141 (1989) | (3) J.C. Vera et al.; Anal. Biochem 174, 38 (1988) | (4) E.T. Thean et al.; Anal. Biochem 177, 263 (1989) | (5) D.S. Perlin et al.; J. Biol. Chem 259, 9532 (1984) | (6) K.G. Mann et al.; Meth. Enzymol. 26, 28 (1972)