Zin3 AM

CHF 255.00
In stock
CDX-F0019-C100100 µgCHF 255.00
CDX-F0019-C500500 µgCHF 1'021.00
CDX-F0019-M0011 mgCHF 1'786.00
More Information
Product Details
Synonyms FluoZin-3 AM (Registered Trademark of ThermoFisher)
Product Type Chemical
Properties
Formula

C46H44F2N2O20

MW 982.85
CAS 562102-46-9
Source/Host Chemicals Synthetic.
Purity Chemicals ≥85% (HPLC)
Appearance Orange to red solid.
Solubility Soluble in DMSO or methanol.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key KMLHLVARODXKDJ-UHFFFAOYSA-N
Smiles O=C1C(F)=CC2=C(C3=CC=C(NCC(OCOC(C)=O)=O)C(OCCOC4=CC(OC)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)C5=CC(F)=C(OCOC(C)=O)C=C5OC2=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Zin3 AM is a cell-permeable Zn2+-selective indicator (Absorption=456nm), that once it enters the cell is hydrolized by esterases to convert the bright fluorescent Zin3 (Ex/Em: ~494/516nm). Zin3 is suitable for detection of Zn2+ concentrations in the 1–100 nM range and it has shown to be very Zn2+-sensitive and Zn2+-specific. The cell permeant AM-ester is useful for detecting low intracellular Zn2+ levels and small concentration changes. Zin3 exhibits high Zn2+-binding affinity that is unperturbed by Ca2+ concentrations up to at least 1 µM (Kd (Zn2+) (in buffer): ~15nM). In addition, Zin3 exhibits a >50-fold increase in fluorescence in response to saturating levels of Zn2+. This dye is usedpredominantly for imaging applications, but has also used in cell-based microplate assays and flow cytometry protocols. The product is typically loaded into cells by adding the dissolved indicator to medium containing cells.

Zinc is an important divalent cation in biological systems, influencing DNA synthesis, microtubule polymerization, gene expression, apoptosis, immune system function and the activity of enzymes such as carbonic anhydrase and matrix metalloproteinases (MMP). Zn2+ is also functionally active in synaptic transmission, and is a contributory factor in neurological disorders including epilepsy and Alzheimer’s disease.

Product References

(1) K.R. Gee, et al.; Cell Calcium 31, 245 (2002) | (2) K.R. Gee, et al.; JACS 124, 776 (2002) | (3) W.J. Qian, et al.; Biotechniques 37, 922 (2004) | (4) M.J. Devinney, et al.; Cell Calcium 37, 225 (2005) | (5) F.A. Muylle, et al.; Biometals 19, 437 (2006) | (6) H. Haase, et al.; Anal. Biochem. 352, 222 (2006) | (7) Z.L. Datki, et al.; Brain Res. Bull. 74, 183 (2007) | (8) J. Zhao, et al.; Cell Calcium 44, 422 (2008) | (9) J. Zhao, et al.; Anal. Biochem. 384, 34 (2009) | (10) Y.M. Li, et al.; Cell Biochem. Funct. 27, 417 (2009) | (11) M.A. Namdarghanbari, et al.; J. Inorg. Biochem. 104, 224 (2010) | (12) R.W. Sabnis; Handbook of biological dyes and stains (2010) | (13) I. Marszalek, et al.; J. Inorg. Biochem. 161, 107 (2016) | (14) I. Marszalek, et al.; Inorg. Chem. 57, 9826 (2019) | (15) L.M. Malaiyandi, et al.; Biometals (Epub ahead of print) (2019)

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