Chemodex

Gram's Crystal Violet Solution

CHF 34.00
In stock
CDX-G0060-L250250 mlCHF 34.00
CDX-G0060-LL252.5 lCHF 217.00
More Information
Product Details
Product Type Chemical
Properties
CAS 548-62-9 (main comp.)
Source/Host Chemicals Synthetic.
Purity Chemicals for microscopy
Appearance Purple to very dark purple liquid.
Solubility Soluble in water.
Identity Determined by UV/Vis.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key ZXJXZNDDNMQXFV-UHFFFAOYSA-M
Smiles CN(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C/3)=[N+](C)/C)C=C1.[Cl-]
Shipping and Handling
Shipping AMBIENT
Short Term Storage +20°C
Long Term Storage +20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at RT.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Gram Crystal Violet Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.

Product References

(1) E. Adams; Stain Technol. 50, 227 (1975) | (2) R. Coico, et al.; Curr. Protoc. Microbiol. Appendix 3-3C (2005)

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.