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Chemodex
4-Methylumbelliferyl-β-D-glucopyranoside
As low as
110
CHF
CHF 110.00
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CDX-M0099-G0011 gCHF 110.00
CDX-M0099-G0055 gCHF 451.00
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| Product Details | |
|---|---|
| Synonyms | MUD; 4-MU-β-D-Glc; 4-Methylumbelliferyl-β-D-glucoside |
| Product Type | Chemical |
| Properties | |
| Formula | C16H18O8 |
| MW | 338.31 |
| CAS | 18997-57-4 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥97% (NMR) |
| Appearance | White to off-white powder. |
| Solubility | Soluble in DMSO or DMF. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | YUDPTGPSBJVHCN-YMILTQATSA-N |
| Smiles | O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@@H](O)[C@@H]1O |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
4-Methylumbelliferyl-β-D-glucopyranoside (MUD) is a non-fluorescent but sensitive fluorogenic substrate for β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is employed in the detection of β-glucosidase as an indicator of Enterococci. It has been extensively used in work on Gaucher's disease, a lipid storage disease characterized by the accumulation of glucocerebroside due to a genetic deficiency of a β-glucosidase. The use of 4-Methylumbelliferyl-β-D-glucopyranoside has also been reported in a rapid method for identifying bacterial enzymes. Hydrolysis of MUD releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).
Product References
(1) S.P. Peters, et al.; Clin. Chim. Acta 60, 391 (1975) | (2) D.M. Broadhead & J. Butterworth; Clin. Chim. Acta 75, 155 (1977) | (3) A. Basu & R.H. Glew; J. Biol. Chem. 260, 13067 (1985) | (4) K.J. Panosian & S.C. Edberg; J. Clin. Microbiol. 27, 1719 (1989) | (5) S. Nakagawa, et al.; Clin. Chim. Acta. 118, 99 (1992) | (6) B. Setlow, et al.; J. Appl. Microbiol. 96, 1245 (2004) | (7) R. Kwapiszewski, et al.; Biomed. Microdevices 13, 431 (2011) | (8) M. Shanmuganathan & P. Britz-McKibbin; Anal. Bioanal. Chem. 399, 2843 (2011) | (9) L. Oftedal, et al.; Sci. Rep. 10, 22098 (2020)