4-Methylumbelliferyl-β-D-glucopyranoside

4-Methylumbelliferyl-β-D-glucopyranoside (MUD) is a non-fluorescent but sensitive fluorogenic substrate for β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is employed in the detection of β-glucosidase as an indicator of Enterococci. It has been extensively used in work on Gaucher's disease, a lipid storage disease characterized by the accumulation of glucocerebroside due to a genetic deficiency of a β-glucosidase. The use of 4-Methylumbelliferyl-β-D-glucopyranoside has also been reported in a rapid method for identifying bacterial enzymes. Hydrolysis of MUD releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).