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Chemodex
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide
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155
CHF
CHF 155.00
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CDX-M0580-M500500 mgCHF 155.00
CDX-M0580-G0011 gCHF 232.00
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| Product Details | |
|---|---|
| Synonyms | 4-MUF-NAG; 4-MU-GlcNAc; 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside; 4-Methylumbelliferyl-N-acetylglucosaminide |
| Product Type | Chemical |
| Properties | |
| Formula | C18H21NO8 |
| MW | 379.36 |
| CAS | 37067-30-4 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥98% (NMR) |
| Appearance | White powder. |
| Solubility | Soluble in DMSO or DMF. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | QCTHLCFVVACBSA-JVNHZCFISA-N |
| Smiles | O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@@H]1CO |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a non-fluorescent but fluorogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting NAGase, chitinase, chitobiase and N-acetylhexosaminidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. Hydrolysis of 4-MUF-NAG releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).
Product References
(1) P. Chow & B. Weissmann; Carbohydr. Res. 96, 87 (1981) | (2) S. Linko-Lopponen & M. Makinen; Anal. Biochem. 148, 50 (1985) | (3) N.N. Dewji, et al.; Biochem J. 234, 157 (1986) | (4) M. O'Brien & R.R. Colwell; Appl. Environ. Microbiol. 53, 1718 (1987) | (5) P.W. Mueller, et al.; J. Anal. Toxicol. 13, 188 (1989) | (6) B. el Moudni, et al.; Exp. Parasitol. 83, 167 (1996) | (7) H. Li, et al.; Appl. Microbiol. Biotechnol. 60, 420 (2002) | (8) J.L. Guthrie, et al.; Can. J. Microbiol. 51, 491 (2005) | (9) S. Litzinger, et al.; J. Biol. Chem. 285, 35675 (2010) | (10) E. Liebminger, et al.; Test 286, 10793 (2011) | (11) O. Li, et al.; Gene Ther. 27, 226 (2020) | (12) S. Vutharadhi & S.K. Nadimpalli; Plant Physiol. Biochem. 197, 107663 (2023)