Chemodex

Neutral Red 5

CHF 68.00
In stock
CDX-N0249-G02525 gCHF 68.00
CDX-N0249-G100100 gCHF 162.00
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Product Details
Synonyms C.I. 50040; 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; Basic Red 5; Toluylene red
Product Type Chemical
Properties
Formula C15H17CIN4
MW 288.78
CAS 553-24-2
RTECS SG1400000
Source/Host Chemicals Synthetic
Purity Chemicals for microscopy
Appearance Red to dark red powder.
Solubility Soluble in water (10mg/ml).
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key GIBXNAFNURIQII-UHFFFAOYSA-N
Smiles CC1=C(N)C=C(NC(C2=N3)=C/C(C=C2)=[N+](C)/C)C3=C1.[Cl-]
Shipping and Handling
Shipping AMBIENT
Short Term Storage +20°C
Long Term Storage +20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at RT.
Documents
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Product Specification Sheet
Datasheet Download PDF
Description

Neutral red acts as a cellular pH indicator, changing from red to yellow between pH 6.8 and 8.0. More important, it is a dye used for staining in histology. It stains lysosomes red and is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. It is applied in histology for nuclear staining, fluorescence staining, polychrome staining or for counter staining in Gram staining. It has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evalutaion of synthetic and naturally derived biomaterials. In microbiology, it is used in the MacConkey agar to differentiate bacteria for lactose fermentation. Neutral red is often used as a vital stain. Live cells incorporate neutral red into their lysosomes. As cells begin to die, their ability to incorporate neutral red diminishes. Thus, loss of neutral red uptake corresponds to loss of cell viability. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470nm and emission maximum at 580nm. Neutral red is added to some growth media for bacterial and cell cultures. Neutral red can be electrochemically polymerized and used as a redox mediator.

Product References

(1) J. Winckler; Red. Prog. Histochem. Cytochem. 6, 1 (1974) | (2) P. Antal, et al.; Ann. Hematol. 70 259 (1995) | (3) V. Ricci, et al.; BBRC 292, 167 (2002) | (4) Y. Ni, et al.; Anal. Biochem. 352, 231 (2006) | (5) C.D. Link, et al.; J. Biol. Chem. 281, 1808 (2006) | (6) G. Repetto, et al.; Nat. Protoc. 3, 1125 (2008) | (7) M.A. Rauf, et al.; Chem. Cent. J. 2, 19 (2008) | (8) B. Chazotte; Cold Spring Harb. Protoc. 2011, pdb.prot5570 (2011) | (9) G. Ates, et al.; Methods Mol. Biol. 1601, 19 (2017) | (10) L. Xu, et al.; Chem. Commun. 52, 14330 (2016)

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