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Chemodex
Resorufin benzyl ether
84
CHF
CHF 84.00
In stock
CDX-R0014-M0055 mgCHF 84.00
CDX-R0014-M01010 mgCHF 129.00
| Product Details | |
|---|---|
| Synonyms | Benzyloxyresorufin; 7-Benzyloxyresorufin; O7-Benzylresorufin; 7-Benzyloxy-3H-phenoxazin-3-one |
| Product Type | Chemical |
| Properties | |
| Formula | C19H13NO3 |
| MW | 303.31 |
| CAS | 87687-02-3 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥98% (HPLC) |
| Appearance | Orange to red powder. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | XNZRYTITWLGTJS-UHFFFAOYSA-N |
| Smiles | O=C1C=C2OC3=CC(OCC4=CC=CC=C4)=CC=C3N=C2C=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Resorufin benzyl ether (7-Benzyloxyresorufin) is a fluorogenic substrate widely used for monitoring cytochrome P450 (CYP450) activities in cell extracts and solutions. It is the model substrate for 7-Benzoxyresorufin-O-dealkylase (BROD) assays. It is typically used near its apparent Km value of 30 µM to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Cytochrome P450 oxidase is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site. Upon enzymatic cleavage of 7-Benzyloxyresorufin by CYP450, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: λex=571nm, λem=585nm.
Product References
(1) R.T Mayer, at al.; Biochem. Pharmacol. 40, 1645 (1990) | (2) D.M. Stresser, et al.; Drug Metab. Dispos. 28, 1440 (2000) | (3) A.B. Renwick, et al.; Xenobiotica 31, 861 (2001) | (4) D.M. Stresser, et al.; Drug Metab. Dispos. 30, 845 (2002) | (5) B.M.A. Lussenburg, et at.; Anal. Biochem. 341, 148 (2005) | (6) J.R. Reed, et al.; Biochem. Pharmacol. 95, 126 (2015) | (7) J. Reinen, et al., J. Biomol. Screen. 20, 1246 (2015) | (8) M.J. Traylor, et al.; Insect Biochem. Mol. Biol. 90, 14 (2017) | (9) L. Rutz, et al.; Ach. Toxicol. 94, 4159 (2020)