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Chemodex
Resorufin acetate
32
CHF
CHF 32.00
In stock
CDX-R0020-M0055 mgCHF 32.00
CDX-R0020-M02525 mgCHF 129.00
| Product Details | |
|---|---|
| Synonyms | O-acetyl Resorufin; 7-Acetyl-3H-phenoxazin-3-one |
| Product Type | Chemical |
| Properties | |
| Formula | C14H9NO4 |
| MW | 255.23 |
| CAS | 1152-14-3 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥98% (TLC) |
| Appearance | Orange to dark orange powder. |
| Solubility | Soluble in DMSO (5mg/ml) or DMF (20mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | UJWKHDKBOVPINX-UHFFFAOYSA-N |
| Smiles | O=C1C=C2OC3=CC(OC(C)=O)=CC=C3N=C2C=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Resorufin acetate is a fluorogenic substrate for hydrolytic esterases and cellulases used for monitoring cytosolic aldehyde dehydrogenase (ALDH1A1) esterase activity, chymotrypsin activity. Resorufin acetate has been used as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase (GOD), without significant effects of ascorbic acid, uric acid, or bilirubin. It also has been used for the chromogenic and fluorescent detection of hydrazine, acyl protein thioesterase (APT) enzymes and perborate ions, and reveals a selective turn-on type chromogenic and fluorogenic signaling behavior based on the selective and efficient cleavage of acetate group. Upon enzymatic cleavage of the acetate group of resorufin acetate, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: λex=571nm, λem=585nm.
Product References
(1) G.G. Guilbault & A.N.J. Heyn; Anal. Lett. 1, 163 (1967) | (2) T.M. Kitson & K.E. Kitson; Biochem J. 322, 701 (1997) | (3) T.M. Kitson & K.E. Kitson; Adv. Exp. Med. Biol. 414, 201 (1997) | (4) T.M. Kitson; Biochim. Biophys. Acta 1385, 43 (1998) | (5) H. Maeda, et al.; Chem. Pharm. Bull. 49, 294 (2001) | (6) M.G. Choi, et al.; Org. Lett. 12, 1468 (2010) | (7) M.G. Choi, et al.; Org. Biomol. Chem. 11, 2961 (2013)